4W5V
Crystal structure of Human SUMO E2-conjugating enzyme (Ubc9) in complex with E1-activating enzyme (Uba2) ubiquitin fold domain (Ufd)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Detector technology | PIXEL |
| Collection date | 2014-01-24 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97945 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 161.273, 35.272, 58.672 |
| Unit cell angles | 90.00, 96.69, 90.00 |
Refinement procedure
| Resolution | 44.700 - 2.500 |
| R-factor | 0.2244 |
| Rwork | 0.221 |
| R-free | 0.28280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ong 1u9b |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.500 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 44.700 |
| High resolution limit [Å] | 2.500 |
| Number of reflections | 10654 |
| <I/σ(I)> | 9.32 |
| Completeness [%] | 91.1 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 100 mM Tris, pH 8.0, 20% PEG MME 550, 200 mM potassium formate, 20% glycerol; seeded |
