4W5O
The Crystal Structure of Human Argonaute2 Bound to a Guide and Target RNA Containing Seed Pairing from 2-9
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Protein argonaute-2 | polymer | 859 | 97378.2 | 1 | UniProt (Q9UKV8) Pfam (PF16486) Pfam (PF08699) Pfam (PF02170) Pfam (PF16488) Pfam (PF16487) Pfam (PF02171) | Homo sapiens (Human) | hAgo2,Argonaute RISC catalytic component 2,Eukaryotic translation initiation factor 2C 2,eIF2C 2,PAZ Piwi domain protein,PPD,Protein slicer |
| 2 | B (B) | RNA (5'-R(P*UP*UP*CP*AP*CP*AP*UP*UP*GP*CP*CP*CP*AP*AP*GP*UP*CP*UP*UP*U)-3') | polymer | 21 | 6547.9 | 1 | synthetic construct | ||
| 3 | C (D) | RNA (5'-R(*CP*AP*AP*UP*GP*UP*GP*AP*AP*A)-3') | polymer | 11 | 3538.2 | 1 | synthetic construct | ||
| 4 | D, K, M (A, B, D) | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) | |||
| 5 | E, F (A) | ISOPROPYL ALCOHOL | non-polymer | 60.1 | 2 | Chemie (IPA) | |||
| 6 | G, H, I, J (A) | PHENOL | non-polymer | 94.1 | 4 | Chemie (IPH) | |||
| 7 | L (B) | PHOSPHATE ION | non-polymer | 95.0 | 1 | Chemie (PO4) | |||
| 8 | N, O, P (A, B, D) | water | water | 18.0 | 433 | Chemie (HOH) |
Sequence modifications
A: 1 - 859 (UniProt: Q9UKV8)
| PDB | External Database | Details |
|---|---|---|
| Asp 387 | Ser 387 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 107464.3 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 664.5 | |
| All* | Total formula weight | 108128.8 |
