4W5K

Structure of a mitochondrial aspartate aminotransferase from Trypanosoma brucei, K237A mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Aspartate aminotransferase, mitochondrial	polymer	396	43819.1	2	UniProt (Q385Q9) Pfam (PF00155)	Trypanosoma brucei brucei
2	C, F (A, B)	PYRIDOXAL-5'-PHOSPHATE	non-polymer		247.1	2	Chemie (PLP)
3	D, G (A, B)	D-MALATE	non-polymer		134.1	2	Chemie (MLT)
4	E, H (A, B)	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
5	I, J (A, B)	water	water		18.0	794	Chemie (HOH)

Sequence modifications

A, B: 1 - 388 (UniProt: Q385Q9)

PDB	External Database	Details
Met -7	-	expression tag
Ala -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Leu 147	Phe 147	conflict
Ala 237	Lys 237	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	87638.3
Non-Polymers*	Number of molecules	6
	Total formula weight	886.6
All*	Total formula weight	88524.9

*Water molecules are not included.