4V9N

Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2.

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, DB (AA, CA)	16S rRNA (1504-MER)	polymer	1504	488431.2	2	GenBank (AE017221.1)	Thermus thermophilus
2	B, EB (AV, CV)	messenger RNA (5'-R(*AP*AP*UP*GP*UP*AP*G)-3')	polymer	10	3217.0	2		Thermus thermophilus
3	C, FB (AW, CW)	P-site tRNA-fMet	polymer	77	24802.8	2	GenBank (CP001509.3)	Thermus thermophilus
4	D, GB (AY, CY)	Bacterial peptide chain release factor 2 (RF-2)	polymer	362	40889.2	2	UniProt (Q72GJ6)	Thermus thermophilus
5	E, HB (AB, CB)	30S ribosomal protein S2	polymer	234	26987.3	2	UniProt (P62662)	Thermus thermophilus
6	F, IB (AC, CC)	30S ribosomal protein S3	polymer	206	22862.4	2	UniProt (P62663)	Thermus thermophilus
7	G, JB (AD, CD)	30S ribosomal protein S4	polymer	208	24242.3	2	UniProt (P62664)	Thermus thermophilus
8	H, KB (AE, CE)	30S ribosomal protein S5	polymer	151	16460.2	2	UniProt (P62665)	Thermus thermophilus
9	I, LB (AF, CF)	30S ribosomal protein S6	polymer	101	11988.8	2	UniProt (P62666)	Thermus thermophilus
10	J, MB (AG, CG)	30S ribosomal protein S7	polymer	155	17919.8	2	UniProt (P62667)	Thermus thermophilus
11	K, NB (AH, CH)	30S ribosomal protein S8	polymer	138	15868.6	2	UniProt (P62668)	Thermus thermophilus
12	L, OB (AI, CI)	30S ribosomal protein S9	polymer	127	14298.5	2	UniProt (P62669)	Thermus thermophilus
13	M, PB (AJ, CJ)	30S ribosomal protein S10	polymer	98	11299.2	2	UniProt (P62653)	Thermus thermophilus
14	N, QB (AK, CK)	30S ribosomal protein S11	polymer	114	12014.7	2	UniProt (P62654)	Thermus thermophilus
15	O, RB (AL, CL)	30S ribosomal protein S12	polymer	122	13604.1	2	UniProt (P61941)	Thermus thermophilus
16	P, SB (AM, CM)	30S ribosomal protein S13	polymer	117	13308.5	2	UniProt (P62655)	Thermus thermophilus
17	Q, TB (AN, CN)	30S ribosomal protein S14	polymer	60	7027.5	2	UniProt (P62656)	Thermus thermophilus
18	R, UB (AO, CO)	30S ribosomal protein S15	polymer	88	10447.2	2	UniProt (P62657)	Thermus thermophilus
19	S, VB (AP, CP)	30S ribosomal protein S16	polymer	83	9924.5	2	UniProt (P62238)	Thermus thermophilus
20	T, WB (AQ, CQ)	30S ribosomal protein S17	polymer	99	11721.9	2	UniProt (P62658)	Thermus thermophilus
21	U, XB (AR, CR)	30S ribosomal protein S18	polymer	70	8155.8	2	UniProt (P62659)	Thermus thermophilus
22	V, YB (AS, CS)	30S ribosomal protein S19	polymer	78	8949.4	2	UniProt (P62660)	Thermus thermophilus
23	W, ZB (AT, CT)	30S ribosomal protein S20	polymer	99	10907.1	2	UniProt (P62661)	Thermus thermophilus
24	AC, X (AU, CU)	30S ribosomal protein Thx	polymer	24	2960.5	2	UniProt (P62613)	Thermus thermophilus
25	BC, Y (BA, DA)	23S ribosomal RNA	polymer	2879	936302.1	2	GenBank (AE017221.1)	Thermus thermophilus
26	CC, Z (BB, DB)	5S ribosomal RNA	polymer	119	38553.0	2	GenBank (AE017221.1)	Thermus thermophilus
27	AA, DC (BD, DD)	50S ribosomal protein L2	polymer	271	29828.6	2	UniProt (Q72I07)	Thermus thermophilus
28	BA, EC (BE, DE)	50S ribosomal protein L3	polymer	204	22250.0	2	UniProt (Q72I04)	Thermus thermophilus
29	CA, FC (BF, DF)	50S ribosomal protein L4	polymer	202	22487.0	2	UniProt (Q72I05)	Thermus thermophilus
30	DA, GC (BG, DG)	50S ribosomal protein L5	polymer	181	20944.4	2	UniProt (Q72I16)	Thermus thermophilus
31	EA, HC (BH, DH)	50S ribosomal protein L6	polymer	159	17363.1	2	UniProt (Q72I19)	Thermus thermophilus
32	FA, IC (BI, DI)	50S ribosomal protein L9	polymer	145	16107.9	2	UniProt (Q72GV5)	Thermus thermophilus
33	GA, JC (BK, DK)	50S ribosomal protein L11	polymer	147	15526.1	2	UniProt (P62442)	Thermus thermophilus
34	HA, KC (BN, DN)	50S ribosomal protein L13	polymer	137	15568.5	2	UniProt (Q72IN1)	Thermus thermophilus
35	IA, LC (BO, DO)	50S ribosomal protein L14	polymer	122	13309.6	2	UniProt (Q72I14)	Thermus thermophilus
36	JA, MC (BP, DP)	50S ribosomal protein L15	polymer	146	15858.5	2	UniProt (Q72I23)	Thermus thermophilus
37	KA, NC (BQ, DQ)	50S ribosomal protein L16	polymer	134	15148.8	2	UniProt (Q72I11)	Thermus thermophilus
38	LA, OC (BR, DR)	50S ribosomal protein L17	polymer	117	13619.0	2	UniProt (Q72I33)	Thermus thermophilus
39	MA, PC (BS, DS)	50S ribosomal protein L18	polymer	98	10941.9	2	UniProt (Q72I20)	Thermus thermophilus
40	NA, QC (BT, DT)	50S ribosomal protein L19	polymer	137	16247.9	2	UniProt (Q72JU9)	Thermus thermophilus
41	OA, RC (BU, DU)	50S ribosomal protein L20	polymer	117	13648.1	2	UniProt (Q72L76)	Thermus thermophilus
42	PA, SC (BV, DV)	50S ribosomal protein L21	polymer	101	11069.2	2	UniProt (Q72HR2)	Thermus thermophilus
43	QA, TC (BW, DW)	50S ribosomal protein L22	polymer	112	12678.9	2	UniProt (Q72I09)	Thermus thermophilus
44	RA, UC (BX, DX)	50S ribosomal protein L23	polymer	92	10273.1	2	UniProt (Q72I06)	Thermus thermophilus
45	SA, VC (BY, DY)	50S ribosomal protein L24	polymer	100	11078.5	2	UniProt (Q72I15)	Thermus thermophilus
46	TA, WC (BZ, DZ)	50S ribosomal protein L25	polymer	187	21021.1	2	UniProt (Q72IA7)	Thermus thermophilus
47	UA, XC (B0, D0)	50S ribosomal protein L27	polymer	76	8587.9	2	UniProt (Q72HR3)	Thermus thermophilus
48	VA, YC (B1, D1)	50S ribosomal protein L28	polymer	88	9884.7	2	UniProt (Q72G84)	Thermus thermophilus
49	WA, ZC (B2, D2)	50S ribosomal protein L29	polymer	62	7457.9	2	UniProt (Q72I12)	Thermus thermophilus
50	AD, XA (B3, D3)	50S ribosomal protein L30	polymer	59	6670.0	2	UniProt (Q72I22)	Thermus thermophilus
51	BD, YA (B4, D4)	50S ribosomal protein L31	polymer	30	3278.9	2	UniProt (Q72JR0)	Thermus thermophilus
52	CD, ZA (B5, D5)	50S ribosomal protein L32	polymer	52	5818.9	2	UniProt (P62652)	Thermus thermophilus
53	AB, DD (B6, D6)	50S ribosomal protein L33	polymer	44	5473.4	2	UniProt (Q72GW3)	Thermus thermophilus
54	BB, ED (B7, D7)	50S ribosomal protein L34	polymer	48	5975.3	2	UniProt (P80340) Pfam (PF00468)	Thermus thermophilus
55	CB, FD (B8, D8)	50S ribosomal protein L35	polymer	63	7245.9	2	UniProt (Q72L77)	Thermus thermophilus
56	AAA, AAB, AAC, ABA, ABB... (BA, BB, DA, CA, AA...)	MAGNESIUM ION	non-polymer		24.3	2410	Chemie (MG)
57	AOB, DOB, GT, IT (CD, CN, AD, AN)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)

Sequence modifications

AY, CY: 17 - 378 (UniProt: Q72GJ6)

PDB	External Database	Details
Pro 253	Gln 253	engineered mutation

BA, DA: 6 - 2897 (GenBank: AE017221.1)

PDB	External Database	Details
C 276	A 1534789	conflict
A 277	C 1534790	conflict
U 1141	C 1535654	conflict
U 2825	G 1537301	conflict

Contents of the asymmetric unit

Polymers	Number of chains	110
	Total formula weight	4377013.0
Non-Polymers*	Number of molecules	2414
	Total formula weight	58836.7
All*	Total formula weight	4435849.7

*Water molecules are not included.