4V9N
Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2.
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Collection date | 2012-08-02 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 212.070, 454.400, 618.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.672 - 3.400 |
| R-factor | 0.235 |
| Rwork | 0.234 |
| R-free | 0.26810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3F1G AND 3F1H |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.621 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.400 |
| Number of reflections | 807927 |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7.0 | ||
| 1 | 7 | pH 7.0 | ||
| 1 | 7 | pH 7.0 | ||
| 1 | 7 | pH 7.0 |
