4V97

Crystal structure of the bacterial ribosome ram mutation G299A.

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, FB (AA, CA)	16S rRNA	polymer	1522	493942.3	2	GenBank (AP008226.1)	Thermus thermophilus
2	B, GB (AB, CB)	30S ribosomal protein S2	polymer	256	29317.7	2	UniProt (P80371) Pfam (PF00318)	Thermus thermophilus
3	C, HB (AC, CC)	30S ribosomal protein S3	polymer	239	26751.1	2	UniProt (P80372) Pfam (PF07650) Pfam (PF00189)	Thermus thermophilus
4	D, IB (AD, CD)	30S ribosomal protein S4	polymer	209	24373.4	2	UniProt (P80373) Pfam (PF00163) Pfam (PF01479)	Thermus thermophilus
5	E, JB (AE, CE)	30S ribosomal protein S5	polymer	162	17583.4	2	UniProt (Q5SHQ5) Pfam (PF00333) Pfam (PF03719)	Thermus thermophilus
6	F, KB (AF, CF)	30S ribosomal protein S6	polymer	101	11988.8	2	UniProt (Q5SLP8) Pfam (PF01250)	Thermus thermophilus	TS9
7	G, LB (AG, CG)	30S ribosomal protein S7	polymer	156	18051.0	2	UniProt (P17291) Pfam (PF00177)	Thermus thermophilus
8	H, MB (AH, CH)	30S ribosomal protein S8	polymer	138	15868.6	2	UniProt (Q5SHQ2)	Thermus thermophilus
9	I, NB (AI, CI)	30S ribosomal protein S9	polymer	128	14410.6	2	UniProt (P80374) Pfam (PF00380)	Thermus thermophilus
10	J, OB (AJ, CJ)	30S ribosomal protein S10	polymer	105	11955.0	2	UniProt (Q5SHN7) Pfam (PF00338)	Thermus thermophilus
11	K, PB (AK, CK)	30S ribosomal protein S11	polymer	129	13737.9	2	UniProt (P80376) Pfam (PF00411)	Thermus thermophilus
12	L, QB (AL, CL)	30S ribosomal protein S12	polymer	132	14637.4	2	UniProt (Q5SHN3) Pfam (PF00164)	Thermus thermophilus
13	M, RB (AM, CM)	30S ribosomal protein S13	polymer	126	14338.9	2	UniProt (P80377) Pfam (PF00416)	Thermus thermophilus
14	N, SB (AN, CN)	30S ribosomal protein S14 type Z	polymer	61	7158.7	2	UniProt (Q5SHQ1)	Thermus thermophilus
15	O, TB (AO, CO)	30S ribosomal protein S15	polymer	89	10578.4	2	UniProt (Q5SJ76) Pfam (PF00312)	Thermus thermophilus
16	P, UB (AP, CP)	30S ribosomal protein S16	polymer	88	10410.0	2	UniProt (Q5SJH3) Pfam (PF00886)	Thermus thermophilus
17	Q, VB (AQ, CQ)	30S ribosomal protein S17	polymer	105	12325.7	2	UniProt (Q5SHP7)	Thermus thermophilus
18	R, WB (AR, CR)	30S ribosomal protein S18	polymer	88	10258.3	2	UniProt (Q5SLQ0) Pfam (PF01084)	Thermus thermophilus
19	S, XB (AS, CS)	30S ribosomal protein S19	polymer	93	10605.5	2	UniProt (Q5SHP2) Pfam (PF00203)	Thermus thermophilus
20	T, YB (AT, CT)	30S ribosomal protein S20	polymer	106	11736.1	2	UniProt (P80380) Pfam (PF01649)	Thermus thermophilus
21	U, ZB (AU, CU)	30S ribosomal protein Thx	polymer	27	3350.0	2	UniProt (Q5SIH3) Pfam (PF17070)	Thermus thermophilus	S31
22	AC, V (AV, CV)	P-SITE tRNA fMet	polymer	77	24802.8	2	GenBank (AP012306.1)	Escherichia coli
23	BC, DC, W, Y (AW, AY, CW, CY)	E-SITE TRNA PHE OR A-SITE tRNA Phe	polymer	76	24485.5	4	GenBank (AP012306.1)	Escherichia coli
24	CC, X (AX, CX)	mRNA	polymer	24	7827.8	2
25	EC, Z (BA, DA)	23S rRNA	polymer	2915	947895.4	2	GenBank (AP008226.1)	Thermus thermophilus
26	AA, FC (BB, DB)	5S rRNA	polymer	122	39494.5	2	GenBank (AP008226.1)	Thermus thermophilus
27	BA, GC (BC, DC)	50S RIBOSOMAL PROTEIN L1	polymer	229	24872.7	2	UniProt (Q5SLP7) Pfam (PF00687)	Thermus thermophilus
28	CA, HC (BD, DD)	50S RIBOSOMAL PROTEIN L2	polymer	276	30532.6	2	UniProt (P60405) Pfam (PF00181) Pfam (PF03947)	Thermus thermophilus
29	DA, IC (BE, DE)	50S RIBOSOMAL PROTEIN L3	polymer	206	22450.2	2	UniProt (Q5SHN8) Pfam (PF00297)	Thermus thermophilus
30	EA, JC (BF, DF)	50S RIBOSOMAL PROTEIN L4	polymer	210	23271.9	2	UniProt (Q5SHN9) Pfam (PF00573)	Thermus thermophilus
31	FA, KC (BG, DG)	50S RIBOSOMAL PROTEIN L5	polymer	182	21061.6	2	UniProt (Q5SHQ0) Pfam (PF00281) Pfam (PF00673)	Thermus thermophilus
32	GA, LC (BH, DH)	50S RIBOSOMAL PROTEIN L6	polymer	180	19568.9	2	UniProt (Q5SHQ3)	Thermus thermophilus
33	HA, MC (BI, DI)	50S RIBOSOMAL PROTEIN L9	polymer	148	16422.2	2	UniProt (Q5SLQ1) Pfam (PF01281) Pfam (PF03948)	Thermus thermophilus
34	IA, NC (BN, DN)	50S RIBOSOMAL PROTEIN L13	polymer	140	15927.9	2	UniProt (P60488) Pfam (PF00572)	Thermus thermophilus
35	JA, OC (BO, DO)	50S RIBOSOMAL PROTEIN L14	polymer	122	13323.6	2	UniProt (Q5SHP8) Pfam (PF00238)	Thermus thermophilus
36	KA, PC (BP, DP)	50S RIBOSOMAL PROTEIN L15	polymer	150	16319.1	2	UniProt (Q5SHQ7) Pfam (PF00828)	Thermus thermophilus
37	LA, QC (BQ, DQ)	50S RIBOSOMAL PROTEIN L16	polymer	141	15993.9	2	UniProt (P60489) Pfam (PF00252)	Thermus thermophilus
38	MA, RC (BR, DR)	50S RIBOSOMAL PROTEIN L17	polymer	118	13750.1	2	UniProt (Q9Z9H5) Pfam (PF01196)	Thermus thermophilus
39	NA, SC (BS, DS)	50S RIBOSOMAL PROTEIN L18	polymer	112	12639.8	2	UniProt (Q5SHQ4) Pfam (PF00861)	Thermus thermophilus
40	OA, TC (BT, DT)	50S RIBOSOMAL PROTEIN L19	polymer	146	17188.8	2	UniProt (P60490) Pfam (PF01245)	Thermus thermophilus
41	PA, UC (BU, DU)	50S RIBOSOMAL PROTEIN L20	polymer	118	13779.3	2	UniProt (P60491) Pfam (PF00453)	Thermus thermophilus
42	QA, VC (BV, DV)	50S RIBOSOMAL PROTEIN L21	polymer	101	11069.2	2	UniProt (P60492) Pfam (PF00829)	Thermus thermophilus
43	RA, WC (BW, DW)	50S RIBOSOMAL PROTEIN L22	polymer	113	12808.1	2	UniProt (Q5SHP3) Pfam (PF00237)	Thermus thermophilus
44	SA, XC (BX, DX)	50S RIBOSOMAL PROTEIN L23	polymer	96	10759.8	2	UniProt (Q5SHP0) Pfam (PF00276)	Thermus thermophilus
45	TA, YC (BY, DY)	50S RIBOSOMAL PROTEIN L24	polymer	110	12085.6	2	UniProt (Q5SHP9) Pfam (PF00467) Pfam (PF17136)	Thermus thermophilus
46	UA, ZC (BZ, DZ)	50S RIBOSOMAL PROTEIN L25	polymer	206	23238.6	2	UniProt (Q5SHZ1) Pfam (PF01386) Pfam (PF14693)	Thermus thermophilus
47	AD, VA (B0, D0)	50S RIBOSOMAL PROTEIN L27	polymer	85	9529.1	2	UniProt (P60493) Pfam (PF01016)	Thermus thermophilus
48	BD, WA (B1, D1)	50S RIBOSOMAL PROTEIN L28	polymer	98	11004.2	2	UniProt (P60494) Pfam (PF00830)	Thermus thermophilus
49	CD, XA (B2, D2)	50S RIBOSOMAL PROTEIN L29	polymer	72	8670.3	2	UniProt (Q5SHP6) Pfam (PF00831)	Thermus thermophilus
50	DD, YA (B3, D3)	50S RIBOSOMAL PROTEIN L30	polymer	60	6799.1	2	UniProt (Q5SHQ6) Pfam (PF00327)	Thermus thermophilus
51	ED, ZA (B4, D4)	50S RIBOSOMAL PROTEIN L31	polymer	71	8300.5	2	UniProt (Q5SJE1) Pfam (PF01197)	Thermus thermophilus
52	AB, FD (B5, D5)	50S RIBOSOMAL PROTEIN L32	polymer	60	6722.1	2	UniProt (P80339) Pfam (PF01783)	Thermus thermophilus
53	BB, GD (B6, D6)	50S RIBOSOMAL PROTEIN L33	polymer	54	6632.9	2	UniProt (P35871) Pfam (PF00471)	Thermus thermophilus
54	CB, HD (B7, D7)	50S RIBOSOMAL PROTEIN L34	polymer	49	6132.4	2	UniProt (P80340) Pfam (PF00468)	Thermus thermophilus
55	DB, ID (B8, D8)	50S RIBOSOMAL PROTEIN L35	polymer	65	7506.2	2	UniProt (Q5SKU1) Pfam (PF01632)	Thermus thermophilus
56	EB, JD (B9, D9)	50S RIBOSOMAL PROTEIN L36	polymer	37	4435.4	2	UniProt (Q5SHR2) Pfam (PF00444)	Thermus thermophilus
57	AAA, ABA, ACA, ADA, AE... (CA, DA, AA, BA, DX...)	MAGNESIUM ION	non-polymer		24.3	1033	Chemie (MG)
58	MAA, QH (CA, AA)	PAROMOMYCIN	non-polymer		615.6	2	Chemie (PAR)
59	OAA, RAA, RH, TH (CD, CN, AD, AN)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)

Sequence modifications

AA, CA: 0 - 1544 (GenBank: AP008226.1)

PDB	External Database	Details
A 299	G 131594	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	114
	Total formula weight	4550332.6
Non-Polymers*	Number of molecules	1039
	Total formula weight	26600.0
All*	Total formula weight	4576932.5

*Water molecules are not included.