4V97

Crystal structure of the bacterial ribosome ram mutation G299A.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-22
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	210.459, 447.344, 622.298
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.071 - 3.516
R-factor	0.2119
Rwork	0.210
R-free	0.24930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 2WDG 2WDH 2WDI 2WDJ
RMSD bond length	0.009
RMSD bond angle	1.052
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.7_641)
Refinement software	PHENIX ((phenix.refine: 1.7_641))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.700
High resolution limit [Å]	3.500	3.500
Rmerge	0.438	1.303
Number of reflections	720017
<I/σ(I)>	6.3	1.8
Completeness [%]	99.9	99.9
Redundancy	11.5	9.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl2, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K