4V63

Structural basis for translation termination on the 70S ribosome.

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, EB (AA, CA)	16S RRNA	polymer	1525	494985.9	2	GenBank (155076)	Thermus thermophilus
2	B, D, FB, HB (AZ, AY, CZ, CY)	P and E-site tRNA(fMet)	polymer	77	24802.8	4		Escherichia coli
3	C, GB (AV, CV)	mRNA	polymer	27	8862.5	2
4	E, IB (AB, CB)	30S ribosomal protein S2	polymer	256	29317.7	2	UniProt (P62662)	Thermus thermophilus
5	F, JB (AC, CC)	30S ribosomal protein S3	polymer	239	26751.1	2	UniProt (P62663)	Thermus thermophilus
6	G, KB (AD, CD)	30S ribosomal protein S4	polymer	209	24373.4	2	UniProt (P62664)	Thermus thermophilus
7	H, LB (AE, CE)	30S ribosomal protein S5	polymer	162	17583.4	2	UniProt (P62665)	Thermus thermophilus
8	I, MB (AF, CF)	30S ribosomal protein S6	polymer	101	11988.8	2	UniProt (P62666)	Thermus thermophilus
9	J, NB (AG, CG)	30S ribosomal protein S7	polymer	156	18051.0	2	UniProt (P62667)	Thermus thermophilus
10	K, OB (AH, CH)	30S ribosomal protein S8	polymer	138	15868.6	2	UniProt (P62668)	Thermus thermophilus
11	L, PB (AI, CI)	30S ribosomal protein S9	polymer	128	14429.7	2	UniProt (P62669)	Thermus thermophilus
12	M, QB (AJ, CJ)	30S ribosomal protein S10	polymer	105	11955.0	2	UniProt (P62653)	Thermus thermophilus
13	N, RB (AK, CK)	30S ribosomal protein S11	polymer	129	13737.9	2	UniProt (P62654)	Thermus thermophilus
14	O, SB (AL, CL)	30S ribosomal protein S12	polymer	134	14821.6	2	UniProt (P61941)	Thermus thermophilus
15	P, TB (AM, CM)	30S ribosomal protein S13	polymer	126	14338.9	2	UniProt (P62655)	Thermus thermophilus
16	Q, UB (AN, CN)	30S ribosomal protein S14	polymer	61	7158.7	2	UniProt (P62656)	Thermus thermophilus
17	R, VB (AO, CO)	30S ribosomal protein S15	polymer	89	10578.4	2	UniProt (P62657)	Thermus thermophilus
18	S, WB (AP, CP)	30S ribosomal protein S16	polymer	88	10410.0	2	UniProt (P62238)	Thermus thermophilus
19	T, XB (AQ, CQ)	30S ribosomal protein S17	polymer	105	12324.7	2	UniProt (P62658)	Thermus thermophilus
20	U, YB (AR, CR)	30S ribosomal protein S18	polymer	88	10258.3	2	UniProt (P62659)	Thermus thermophilus
21	V, ZB (AS, CS)	30S ribosomal protein S19	polymer	93	10605.5	2	UniProt (P62660)	Thermus thermophilus
22	AC, W (AT, CT)	30S ribosomal protein S20	polymer	106	11722.1	2	UniProt (P62661)	Thermus thermophilus
23	BC, X (AU, CU)	30S ribosomal protein Thx	polymer	27	3350.0	2	UniProt (P62613)	Thermus thermophilus
24	CC, Y (AX, CX)	Peptide chain release factor 1	polymer	354	40153.5	2	UniProt (Q72HB8)	Thermus thermophilus	RF1
25	GD, Z (BA, DA)	23S RRNA	polymer	2894	941050.9	2		Thermus thermophilus
26	AA, HD (BB, DB)	5S RRNA	polymer	124	40152.9	2	GenBank (48271)	Thermus thermophilus
27	BA, DC (BD, DD)	50S ribosomal protein L2	polymer	276	30532.6	2	UniProt (Q72I07)	Thermus thermophilus
28	CA, EC (BE, DE)	50S ribosomal protein L3	polymer	206	22450.2	2	UniProt (Q72I04)	Thermus thermophilus
29	DA, FC (BF, DF)	50S ribosomal protein L4	polymer	210	23304.0	2	UniProt (Q72I05)	Thermus thermophilus
30	EA, GC (BG, DG)	50S ribosomal protein L5	polymer	182	21075.6	2	UniProt (Q72I16)	Thermus thermophilus
31	FA, HC (BH, DH)	50S ribosomal protein L6	polymer	180	19568.9	2	UniProt (Q72I19)	Thermus thermophilus
32	GA, IC (BI, DI)	50S ribosomal protein L9	polymer	148	16436.2	2	UniProt (Q72GV5)	Thermus thermophilus
33	HA, JC (BJ, DJ)	50S ribosomal protein L10	polymer	173	18586.6	2	UniProt (Q72GS1)	Thermus thermophilus
34	IA, KC (BN, DN)	50S ribosomal protein L13	polymer	163	18534.0	2	UniProt (Q72IN1)	Thermus thermophilus
35	JA, LC (BO, DO)	50S ribosomal protein L14	polymer	122	13309.6	2	UniProt (Q72I14)	Thermus thermophilus
36	KA, MC (BP, DP)	50S ribosomal protein L15	polymer	150	16319.1	2	UniProt (Q72I23)	Thermus thermophilus
37	LA, NC (BQ, DQ)	50S ribosomal protein L16	polymer	141	15977.9	2	UniProt (Q72I11)	Thermus thermophilus
38	MA, OC (BR, DR)	50S ribosomal protein L17	polymer	118	13750.1	2	UniProt (Q72I33)	Thermus thermophilus
39	NA, PC (BS, DS)	50S ribosomal protein L18	polymer	112	12639.8	2	UniProt (Q72I20)	Thermus thermophilus
40	OA, QC (BT, DT)	50S ribosomal protein L19	polymer	146	17217.9	2	UniProt (Q72JU9)	Thermus thermophilus
41	PA, RC (BU, DU)	50S ribosomal protein L20	polymer	118	13779.3	2	UniProt (Q72L76)	Thermus thermophilus
42	QA, SC (BV, DV)	50S ribosomal protein L21	polymer	101	11069.2	2	UniProt (Q72HR2)	Thermus thermophilus
43	RA, TC (BW, DW)	50S ribosomal protein L22	polymer	113	12808.1	2	UniProt (Q72I09)	Thermus thermophilus
44	SA, UC (BX, DX)	50S ribosomal protein L23	polymer	96	10759.8	2	UniProt (Q72I06)	Thermus thermophilus
45	TA, VC (BY, DY)	50S ribosomal protein L24	polymer	110	12085.6	2	UniProt (Q72I15)	Thermus thermophilus
46	UA, WC (BZ, DZ)	50S ribosomal protein L25	polymer	206	23238.6	2	UniProt (Q72IA7)	Thermus thermophilus	TL5, General stress protein CTC
47	VA, XC (B0, D0)	50S ribosomal protein L27	polymer	85	9501.1	2	UniProt (Q72HR3)	Thermus thermophilus
48	WA, YC (B1, D1)	50S ribosomal protein L28	polymer	98	11032.2	2	UniProt (Q72G84)	Thermus thermophilus
49	XA, ZC (B2, D2)	50S ribosomal protein L29	polymer	72	8670.3	2	UniProt (Q72I12)	Thermus thermophilus
50	AD, YA (B3, D3)	50S ribosomal protein L30	polymer	60	6799.1	2	UniProt (Q72I22)	Thermus thermophilus
51	BD, ZA (B4, D4)	50S ribosomal protein L31	polymer	97	11275.0	2	UniProt (Q72JR0)	Thermus thermophilus
52	AB, CD (B5, D5)	50S ribosomal protein L32	polymer	60	6734.1	2	UniProt (P62652)	Thermus thermophilus
53	BB, DD (B6, D6)	50S ribosomal protein L33	polymer	54	6632.9	2	UniProt (Q72GW3)	Thermus thermophilus
54	CB, ED (B7, D7)	50S ribosomal protein L34	polymer	49	6132.4	2	UniProt (P80340) Pfam (PF00468)	Thermus thermophilus
55	DB, FD (B8, D8)	50S ribosomal protein L35	polymer	65	7506.2	2	UniProt (Q72L77)	Thermus thermophilus
56	AAA, AAB, AAC, ABA, ABB... (BA, CA, DA, AA, AY...)	MAGNESIUM ION	non-polymer		24.3	2577	Chemie (MG)
57	ISB, QR, UQ, VRB (CN, AN, AD, CD)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)

Sequence modifications

AA, CA: -1 - 1546 (GenBank: 155076)

PDB	External Database	Details
G 466	C 1101	conflict

AL, CL: 1 - 134 (UniProt: P61941)

PDB	External Database	Details
Ala 2	-	insertion
Leu 3	-	insertion

BB, DB: 0 - 119 (GenBank: 48271)

PDB	External Database	Details
A -1	-	insertion
U 120	-	insertion
U 121	-	insertion

BF, DF: 6 - 210 (UniProt: Q72I05)

PDB	External Database	Details
Met 1	-	insertion
Lys 2	-	insertion
Glu 3	-	insertion
Val 4	-	insertion
Ala 5	-	insertion

BN, DN: 1 - 163 (UniProt: Q72IN1)

PDB	External Database	Details
Val 2	-	insertion
Lys 3	-	insertion
Ser 4	-	insertion
Ser 5	-	insertion
Leu 6	-	insertion
Ala 7	-	insertion
Phe 8	-	insertion
Leu 9	-	insertion
Arg 10	-	insertion
Gly 11	-	insertion
Pro 12	-	insertion
Pro 13	-	insertion
Ile 14	-	insertion
Pro 15	-	insertion
Arg 16	-	insertion
Gln 17	-	insertion
Glu 18	-	insertion
Gln 19	-	insertion
Arg 20	-	insertion
Arg 21	-	insertion
Ala 22	-	insertion
Leu 23	-	insertion
Val 24	-	insertion

B4, D4: 1 - 97 (UniProt: Q72JR0)

PDB	External Database	Details
Pro 2	-	insertion
Leu 3	-	insertion
Gly 4	-	insertion
Val 5	-	insertion
His 6	-	insertion
Pro 7	-	insertion
Leu 8	-	insertion
Tyr 9	-	insertion
Thr 10	-	insertion
Lys 11	-	insertion
Arg 12	-	insertion
Trp 13	-	insertion
Leu 14	-	insertion
Ala 15	-	insertion
His 16	-	insertion
Gly 17	-	insertion
Gln 18	-	insertion
Asp 19	-	insertion
Arg 20	-	insertion
Ala 21	-	insertion
Lys 22	-	insertion
Lys 23	-	insertion
Glu 24	-	insertion
Ala 25	-	insertion
Asn 26	-	insertion
Val 27	-	insertion

Contents of the asymmetric unit

Polymers	Number of chains	112
	Total formula weight	4564324.7
Non-Polymers*	Number of molecules	2581
	Total formula weight	62895.6
All*	Total formula weight	4627220.3

*Water molecules are not included.