4V63
Structural basis for translation termination on the 70S ribosome.
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-17 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.95370 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 210.130, 454.390, 616.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.901 - 3.207 |
| R-factor | 0.292 |
| Rwork | 0.292 |
| R-free | 0.31900 |
| Starting model (for MR) | PROTEIN DATA BANK ACCESSION CODES 2J00 2J01 2J02 AND 2J03. |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.708 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS (1.2) |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.300 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.271 | 0.795 |
| Number of reflections | 906561 | |
| <I/σ(I)> | 8.21 | 2.85 |
| Completeness [%] | 95.9 | 89 |
| Redundancy | 12.4 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 296 | 100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | TRIS-OAc | ||
| 10 | 1 | 2 | PEG200 | ||
| 11 | 1 | 2 | DEOXY BIGCHAP | ||
| 12 | 1 | 2 | H2O | ||
| 2 | 1 | 1 | KSCN | ||
| 3 | 1 | 1 | PEG20K | ||
| 4 | 1 | 1 | PEG200 | ||
| 5 | 1 | 1 | DEOXY BIGCHAP | ||
| 6 | 1 | 1 | H2O | ||
| 7 | 1 | 2 | TRIS-OAc | ||
| 8 | 1 | 2 | KSCN | ||
| 9 | 1 | 2 | PEG20K |
