4V3L

RNF38-UB-UbcH5B-Ub complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	UBIQUITIN-CONJUGATING ENZYME E2 D2	polymer	146	16650.1	1	UniProt (P62837) Pfam (PF00179) In PDB	HOMO SAPIENS (HUMAN)	UBIQUITIN CARRIER PROTEIN D2, UBIQUITIN-CONJUGATING ENZYME E2(17)KB 2, UBIQUITIN-CONJUGATING ENZYME E2-17 KDA 2, UBIQUITIN-PROTEIN LIGASE D2, P53-REGULATED UBIQUITIN-CONJUGATING ENZYME 1, UBCH5B
2	B, D	POLYUBIQUITIN-C	polymer	81	8922.1	2	UniProt (P0CG48) Pfam (PF00240) In PDB	HOMO SAPIENS (HUMAN)	UBIQUITIN
3	C	E3 UBIQUITIN-PROTEIN LIGASE RNF38	polymer	79	9135.3	1	UniProt (Q9H0F5) Pfam (PF13639) In PDB	HOMO SAPIENS (HUMAN)	RING FINGER PROTEIN 38, RNF38
4	A	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
5	C	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
6		water	water		18.0	387	Chemie (HOH)

Sequence modifications

A: 2 - 147 (UniProt: P62837)

PDB	External Database	Details
Lys 85	Cys 85	engineered mutation

B, D: 1 - 76 (UniProt: P0CG48)

PDB	External Database	Details
Gly -4	-	expression tag
Ser -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

C: 389 - 465 (UniProt: Q9H0F5)

PDB	External Database	Details
Gly 387	-	expression tag
Ser 388	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	43629.7
Non-Polymers*	Number of molecules	4
	Total formula weight	255.0
All*	Total formula weight	43884.6

*Water molecules are not included.