4V3L
RNF38-UB-UbcH5B-Ub complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-07 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 62.840, 62.840, 191.060 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 63.687 - 1.530 |
| R-factor | 0.1833 |
| Rwork | 0.183 |
| R-free | 0.19260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.233 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.700 | 1.570 |
| High resolution limit [Å] | 1.530 | 1.530 |
| Rmerge | 0.040 | 0.560 |
| Number of reflections | 66991 | |
| <I/σ(I)> | 30.1 | 3.6 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 9.5 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 50 MM HEPES, PH 7.5, 0.2 M MAGNESIUM ACETATE AND 13% (W/V) PEG 8K |
