4V3K

RNF38-UbcH5B-UB complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, D)	UBIQUITIN-CONJUGATING ENZYME E2 D2	polymer	146	16720.2	2	UniProt (P62837) Pfam (PF00179)	HOMO SAPIENS (HUMAN)	UBIQUITIN CARRIER PROTEIN D2, UBIQUITIN-CONJUGATING ENZYME E2(17)KB 2, UBIQUITIN-CONJUGATING ENZYME E2-17 KDA 2, UBIQUITIN-PROTEIN LIGASE D2, P53-REGULATED UBIQUITIN-CONJUGATING ENZYME 1, UBCH5B
2	B, E (B, E)	POLYUBIQUITIN-C	polymer	81	8922.1	2	UniProt (P0CG48) Pfam (PF00240)	HOMO SAPIENS (HUMAN)	UBIQUITIN
3	C, F (C, F)	E3 UBIQUITIN-PROTEIN LIGASE RNF38	polymer	79	9135.3	2	UniProt (Q9H0F5) Pfam (PF13639)	HOMO SAPIENS (HUMAN)	RING FINGER PROTEIN 38, RNF38
4	G, H, I, O, P... (A, D)	CHLORIDE ION	non-polymer		35.5	6	Chemie (CL)
5	J, K, L, R, S (A, B, D, E)	1,2-ETHANEDIOL	non-polymer		62.1	5	Chemie (EDO)
6	M, N, T, U (C, F)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
7	AA, V, W, X, Y... (F, A, B, C, D...)	water	water		18.0	368	Chemie (HOH)

Sequence modifications

A, D: 2 - 147 (UniProt: P62837)

PDB	External Database	Details
Arg 22	Ser 22	engineered mutation
Lys 85	Cys 85	engineered mutation

B, E: 1 - 76 (UniProt: P0CG48)

PDB	External Database	Details
Gly -4	-	expression tag
Ser -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

C, F: 389 - 465 (UniProt: Q9H0F5)

PDB	External Database	Details
Gly 387	-	expression tag
Ser 388	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	69555.3
Non-Polymers*	Number of molecules	15
	Total formula weight	784.7
All*	Total formula weight	70340.0

*Water molecules are not included.