4V3K

RNF38-UbcH5B-UB complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-07-01
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 41 21 2
Unit cell lengths	139.620, 139.620, 70.690
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.905 - 2.040
R-factor	0.1829
Rwork	0.181
R-free	0.22340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 3ZNI AND 1X4J
RMSD bond length	0.008
RMSD bond angle	1.184
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.900	2.090
High resolution limit [Å]	2.040	2.040
Rmerge	0.120	0.820
Number of reflections	44936
<I/σ(I)>	18.4	3.8
Completeness [%]	99.9	99.9
Redundancy	9.4	9.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				50 MM TRIS-HCL, PH 8.5 AND 2.3 M AMMONIUM SULFATE