4UZU
Three-dimensional structure of a variant `Termamyl-like' Geobacillus stearothermophilus alpha-amylase at 1.9 A resolution
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | ALPHA-AMYLASE | polymer | 513 | 58632.2 | 1 | UniProt (P06279) Pfam (PF00128) Pfam (PF09154) In PDB | GEOBACILLUS STEAROTHERMOPHILUS | TERMAMYL AMYLASE, 1\,4-ALPHA-D-GLUCAN GLUCANOHYDROLASE |
| 2 | A | GLYCINE | non-polymer | 75.1 | 2 | Chemie (GLY) | |||
| 3 | A | CALCIUM ION | non-polymer | 40.1 | 3 | Chemie (CA) | |||
| 4 | A | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) | |||
| 5 | A | SODIUM ION | non-polymer | 23.0 | 3 | Chemie (NA) | |||
| 6 | water | water | 18.0 | 406 | Chemie (HOH) |
Sequence modifications
A: 1 - 513 (UniProt: P06279)
| PDB | External Database | Details |
|---|---|---|
| Thr 73 | Ala 107 | conflict |
| - | Gly 214 | deletion |
| - | Ile 215 | deletion |
| Phe 191 | Asn 227 | engineered mutation |
| Asn 215 | Ser 251 | conflict |
| Tyr 248 | Asp 284 | conflict |
| Thr 276 | Met 312 | conflict |
| Asp 279 | Asn 315 | conflict |
| Ala 302 | Thr 338 | conflict |
| Gly 414 | Val 450 | conflict |
| Arg 490 | Trp 526 | conflict |
| Pro 491 | Ser 527 | conflict |
| Gly 499 | Asp 535 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 58632.2 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 410.2 | |
| All* | Total formula weight | 59042.4 |
