4UZU
Three-dimensional structure of a variant `Termamyl-like' Geobacillus stearothermophilus alpha-amylase at 1.9 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 120 |
| Collection date | 2009-10-26 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 108.180, 108.180, 181.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 92.920 - 1.900 |
| R-factor | 0.17196 |
| Rwork | 0.170 |
| R-free | 0.21029 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hvx |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.437 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.210 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.120 | 0.520 |
| Number of reflections | 42693 | |
| <I/σ(I)> | 17.6 | 5.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.7 | 14.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 65% MPD, 0.1 M HEPES PH 8.2 |
