4UYQ

High resolution structure of the third cohesin ScaC in complex with the ScaB dockerin with a mutation in the C-terminal helix (IN to SI) from Acetivibrio cellulolyticus displaying a type I interaction.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Cellulosomal scaffoldin anchoring protein C	polymer	151	16512.2	1	UniProt (Q7WYN2)	Acetivibrio cellulolyticus
2	B (B)	Cellulosomal scaffoldin adaptor protein B	polymer	75	8382.5	1	UniProt (Q7WYN3)	Acetivibrio cellulolyticus
3	C, D, E (A, B)	CALCIUM ION	non-polymer		40.1	3	Chemie (CA)
4	F, G (A, B)	water	water		18.0	319	Chemie (HOH)

Sequence modifications

A: 2 - 143 (UniProt: Q7WYN2)

PDB	External Database	Details
Met 1	-	initiating methionine
Leu 144	-	expression tag
Glu 145	-	expression tag
His 146	-	expression tag
His 147	-	expression tag
His 148	-	expression tag
His 149	-	expression tag
His 150	-	expression tag
His 151	-	expression tag

B: 1 - 75 (UniProt: Q7WYN3)

PDB	External Database	Details
Ser 51	Ile 918	engineered mutation
Ile 52	Asn 919	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	24894.6
Non-Polymers*	Number of molecules	3
	Total formula weight	120.2
All*	Total formula weight	25014.9

*Water molecules are not included.