4UYQ
High resolution structure of the third cohesin ScaC in complex with the ScaB dockerin with a mutation in the C-terminal helix (IN to SI) from Acetivibrio cellulolyticus displaying a type I interaction.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-01-27 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 43 |
| Unit cell lengths | 68.170, 68.170, 57.140 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 68.170 - 1.810 |
| R-factor | 0.15192 |
| Rwork | 0.150 |
| R-free | 0.19501 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4uyp |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.144 |
| Data reduction software | MOSFLM |
| Data scaling software | FAST_DP |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.170 | 1.880 |
| High resolution limit [Å] | 1.810 | 1.810 |
| Rmerge | 0.110 | 0.420 |
| Number of reflections | 23100 | |
| <I/σ(I)> | 5.35 | 2.01 |
| Completeness [%] | 96.9 | 93 |
| Redundancy | 2.3 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.2 M SODIUM ACETATE 0.1 M HEPES, PH 7.5 1.5 M K2HPO4 1.5 M NAH2PO4 |
