4UXA

Improved variant of (R)-selective manganese-dependent hydroxynitrile lyase from bacteria

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	CUPIN 2 CONSERVED BARREL DOMAIN PROTEIN	polymer	131	14347.0	20	UniProt (E8WYN5) Pfam (PF07883) In PDB	GRANULICELLA TUNDRICOLA	HYDROXYNITRILE LYASE
2	G, H, I, J, K...	MANGANESE (II) ION	non-polymer		54.9	20	Chemie (MN)
3		water	water		18.0	1666	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T: 1 - 131 (UniProt: E8WYN5)

PDB	External Database	Details
His 40	Ala 40	engineered mutation
Thr 42	Val 42	engineered mutation
His 110	Gln 110	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	20
	Total formula weight	286940.0
Non-Polymers*	Number of molecules	20
	Total formula weight	1098.8
All*	Total formula weight	288038.7

*Water molecules are not included.