4UTT

Structural characterisation of NanE, ManNac6P C2 epimerase, from Clostridium perfingens

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	PUTATIVE N-ACETYLMANNOSAMINE-6-PHOSPHATE 2-EPIMERASE	polymer	229	25164.9	2	UniProt (Q0TUP9) Pfam (PF04131) UniProt (by SIFTS) (Q8XNZ3)	CLOSTRIDIUM PERFRINGENS STR. 13	MANNAC-6-P EPIMERASE, N-ACETYLMANNOSAMINE-6-PHOSPHATE 2-EPIMERASE
2	C, D (C, D)	PUTATIVE N-ACETYLMANNOSAMINE-6-PHOSPHATE 2-EPIMERASE	polymer	229	25113.8	2	UniProt (Q0TUP9) Pfam (PF04131) UniProt (by SIFTS) (Q8XNZ3)	CLOSTRIDIUM PERFRINGENS STR. 13	MANNAC-6-P EPIMERASE, N-ACETYLMANNOSAMINE-6-PHOSPHATE 2-EPIMERASE
3	E, F, H, I, K... (A, B, C, D)	CHLORIDE ION	non-polymer		35.5	8	Chemie (CL)
4	G, J, M, P (A, B, C, D)	ACETATE ION	non-polymer		59.0	4	Chemie (ACT)
5	Q, R, S, T (A, B, C, D)	water	water		18.0	1114	Chemie (HOH)

Sequence modifications

A, B: 1 - 220 (UniProt: Q0TUP9)

PDB	External Database	Details
Gly -8	-	expression tag
Ser -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag

C, D: 1 - 220 (UniProt: Q0TUP9)

PDB	External Database	Details
Gly -8	-	expression tag
Ser -7	-	expression tag
Ser -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	100557.5
Non-Polymers*	Number of molecules	12
	Total formula weight	519.8
All*	Total formula weight	101077.3

*Water molecules are not included.