4UT0

THE CRYSTAL STRUCTURE OF I-DMOI IN COMPLEX WITH ITS TARGET DNA AT 10 DAYS INCUBATION IN 5MM MN (STATE 7)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, F, K (A, F, K)	HOMING ENDONUCLEASE I-DMOI	polymer	199	23265.1	3	UniProt (P21505) Pfam (PF14528)	DESULFUROCOCCUS MOBILIS	I-DMOI
2	B, G, L (B, G, L)	5'-D(*GP*CP*CP*TP*TP*GP*CP*CP*GP*GP*GP*TP*AP*AP)-3'	polymer	14	4296.8	3		SYNTHETIC CONSTRUCT
3	C, H, M (C, H, M)	5'-D(*GP*TP*TP*CP*CP*GP*GP*CP*GP*CP*GP)-3'	polymer	11	3366.2	3		SYNTHETIC CONSTRUCT
4	D, I, N (D, I, N)	5'-D(*CP*GP*CP*GP*CP*CP*GP*GP*AP*AP*CP*TP*TP*AP*CP)-3'	polymer	15	4555.0	3		SYNTHETIC CONSTRUCT
5	E, J, O (E, J, O)	5'-D(*CP*CP*GP*GP*CP*AP*AP*GP*GP*CP)-3'	polymer	10	3055.0	3		SYNTHETIC CONSTRUCT
6	P, Q, T, U, W... (A, F, K)	MANGANESE (II) ION	non-polymer		54.9	6	Chemie (MN)
7	R, S, V (A, F)	ACETATE ION	non-polymer		59.0	3	Chemie (ACT)
8	AA, BA, CA, DA, EA... (C, D, E, F, G...)	water	water		18.0	101	Chemie (HOH)

Sequence modifications

A, F, K: 2 - 188 (UniProt: P21505)

PDB	External Database	Details
Ala 1	-	expression tag
Ala 189	-	expression tag
Ala 190	-	expression tag
Ala 191	-	expression tag
Leu 192	-	expression tag
Glu 193	-	expression tag
His 194	-	expression tag
His 195	-	expression tag
His 196	-	expression tag
His 197	-	expression tag
His 198	-	expression tag
His 199	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	15
	Total formula weight	115614.0
Non-Polymers*	Number of molecules	9
	Total formula weight	506.8
All*	Total formula weight	116120.8

*Water molecules are not included.