4UT0

THE CRYSTAL STRUCTURE OF I-DMOI IN COMPLEX WITH ITS TARGET DNA AT 10 DAYS INCUBATION IN 5MM MN (STATE 7)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 1 21 1
Unit cell lengths	106.984, 70.387, 107.154
Unit cell angles	90.00, 119.81, 90.00

Refinement procedure

Resolution	46.489 - 2.400
R-factor	0.1845
Rwork	0.183
R-free	0.22820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vs8
RMSD bond length	0.010
RMSD bond angle	1.269
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.490	2.530
High resolution limit [Å]	2.400	2.400
Rmerge	0.060	0.530
Number of reflections	54032
<I/σ(I)>	12.3	2.3
Completeness [%]	99.4	99
Redundancy	3.7	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6		PH 6