4USJ

N-acetylglutamate kinase from Arabidopsis thaliana in complex with PII from Chlamydomonas reinhardtii

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	ACETYLGLUTAMATE KINASE, CHLOROPLASTIC	polymer	318	33425.6	2	UniProt (Q9SCL7) Pfam (PF00696)	ARABIDOPSIS THALIANA (THALE CRESS)	N-ACETYL-L-GLUTAMATE 5-PHOSPHOTRANSFERASE, NAG KINASE, ATNAGK, ACETYLGLUTAMATE KINASE
2	C, D (C, D)	NITROGEN REGULATORY PROTEIN PII	polymer	154	17071.7	2	UniProt (A8JI83)	CHLAMYDOMONAS REINHARDTII
3	E, I (A, B)	ARGININE	non-polymer		175.2	2	Chemie (ARG)
4	F (A)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)
5	G (A)	N-ACETYL-L-GLUTAMATE	non-polymer		189.2	1	Chemie (NLG)
6	H, L, O (A, C, D)	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
7	J (B)	N-ACETYL-L-GLUTAMYL 5-PHOSPHATE	non-polymer		269.1	1	Chemie (X2W)
8	K, N (C, D)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (ATP)
9	M, P (C, D)	GLUTAMINE	non-polymer		146.1	2	Chemie (GLN)
10	Q, R, S, T (A, B, C, D)	water	water		18.0	104	Chemie (HOH)

Sequence modifications

A, B: 1 - 297 (UniProt: Q9SCL7)

PDB	External Database	Details
Met -20	-	expression tag
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Val -6	-	expression tag
Pro -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag

C, D: 2 - 144 (UniProt: A8JI83)

PDB	External Database	Details
Met 1	-	expression tag
Ser 145	-	expression tag
Ala 146	-	expression tag
Trp 147	-	expression tag
Ser 148	-	expression tag
His 149	-	expression tag
Pro 150	-	expression tag
Gln 151	-	expression tag
Phe 152	-	expression tag
Glu 153	-	expression tag
Lys 154	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	100994.4
Non-Polymers*	Number of molecules	12
	Total formula weight	2615.5
All*	Total formula weight	103609.9

*Water molecules are not included.