4USJ

N-acetylglutamate kinase from Arabidopsis thaliana in complex with PII from Chlamydomonas reinhardtii

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003991	molecular_function	acetylglutamate kinase activity
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0006526	biological_process	arginine biosynthetic process
A	0016301	molecular_function	kinase activity
B	0003991	molecular_function	acetylglutamate kinase activity
B	0005524	molecular_function	ATP binding
B	0005737	cellular_component	cytoplasm
B	0006526	biological_process	arginine biosynthetic process
B	0016301	molecular_function	kinase activity
C	0006808	biological_process	regulation of nitrogen utilization
C	0030234	molecular_function	enzyme regulator activity
D	0006808	biological_process	regulation of nitrogen utilization
D	0030234	molecular_function	enzyme regulator activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	9
Details	BINDING SITE FOR RESIDUE ARG A 301

Chain	Residue
A	LYS210
A	LYS232
A	GLU284
A	ILE285
A	SER287
A	ASP288
A	GLU289
A	GLY292
A	MET294

---

site_id	AC2
Number of Residues	18
Details	BINDING SITE FOR RESIDUE ADP A 302

Chain	Residue
A	LYS41
A	GLY43
A	GLY44
A	ALA45
A	THR215
A	ASP216
A	GLY219
A	LEU221
A	LYS247
A	ALA249
A	GLY251
A	MET252
A	LYS255
A	MG304
A	HOH2006
A	HOH2007
A	HOH2031
B	GLN152

---

site_id	AC3
Number of Residues	10
Details	BINDING SITE FOR RESIDUE NLG A 303

Chain	Residue
A	GLY75
A	GLY76
A	GLY77
A	LEU97
A	ARG98
A	ASN192
A	ASN194
A	ALA195
A	HOH2005
A	HOH2007

---

site_id	AC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MG A 304

Chain	Residue
A	ADP302
A	HOH2005
A	HOH2007
A	HOH2031

---

site_id	AC5
Number of Residues	11
Details	BINDING SITE FOR RESIDUE ARG B 301

Chain	Residue
B	PHE33
B	LYS210
B	LYS232
B	GLU284
B	ILE285
B	SER287
B	ASP288
B	GLU289
B	GLY290
B	GLY292
B	MET294

---

site_id	AC6
Number of Residues	13
Details	BINDING SITE FOR RESIDUE X2W B 303

Chain	Residue
B	LYS41
B	GLY44
B	GLY75
B	GLY76
B	GLY77
B	LEU97
B	ARG98
B	ASN192
B	ASN194
B	ALA195
B	HOH2028
B	HOH2029
B	HOH2030

---

site_id	AC7
Number of Residues	25
Details	BINDING SITE FOR RESIDUE ATP C 301

Chain	Residue
C	ILE24
C	GLY44
C	LEU45
C	THR46
C	GLY52
C	VAL53
C	GLY54
C	GLN56
C	ASP80
C	VAL82
C	ILE104
C	GLY105
C	ASP106
C	GLY107
C	LYS108
C	PHE110
C	ARG119
C	ARG121
C	MET132
C	MG302
C	GLN303
C	HOH2008
C	HOH2009
C	HOH2010
C	HOH2018

---

site_id	AC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MG C 302

Chain	Residue
C	GLY54
C	ATP301
C	HOH2009
C	HOH2010

---

site_id	AC9
Number of Residues	10
Details	BINDING SITE FOR RESIDUE GLN C 303

Chain	Residue
C	GLU130
C	MET132
C	MET136
C	GLU137
C	ATP301
C	ILE42
C	ARG43
C	GLY44
C	VAL82
C	GLN87

---

site_id	BC1
Number of Residues	23
Details	BINDING SITE FOR RESIDUE ATP D 301

Chain	Residue
D	ILE24
D	GLY44
D	LEU45
D	THR46
D	GLY52
D	VAL53
D	GLY54
D	GLN56
D	ASP80
D	VAL82
D	ILE104
D	GLY105
D	ASP106
D	GLY107
D	LYS108
D	ARG119
D	ARG121
D	MET132
D	MG302
D	GLN303
D	HOH2012
D	HOH2013
D	HOH2017

---

site_id	BC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MG D 302

Chain	Residue
D	GLY54
D	ATP301
D	HOH2012
D	HOH2013

---

site_id	BC3
Number of Residues	11
Details	BINDING SITE FOR RESIDUE GLN D 303

Chain	Residue
D	ILE42
D	ARG43
D	GLY44
D	VAL82
D	GLN87
D	GLU130
D	MET132
D	GLY135
D	MET136
D	GLU137
D	ATP301

---

Functional Information from PROSITE/UniProt

site_id	PS00638
Number of Residues	14
Details	PII_GLNB_CTER P-II protein C-terminal region signature. TgeiGDGKIFVhpV

Chain	Residue	Details
C	THR101-VAL114

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	6
Details	BINDING: BINDING => ECO:0000269|PubMed:25416954, ECO:0007744|PDB:4USJ

Chain	Residue	Details
A	GLY44
A	GLY76
A	THR215
B	GLY44
B	GLY76
B	THR215

---

site_id	SWS_FT_FI2
Number of Residues	14
Details	BINDING: BINDING => ECO:0000269|PubMed:17913711, ECO:0000269|PubMed:25416954, ECO:0007744|PDB:2RD5, ECO:0007744|PDB:4USJ

Chain	Residue	Details
A	ARG98
B	LYS210
B	LEU221
B	LYS232
B	GLU284
B	GLY292
A	ASN192
A	LYS210
A	LEU221
A	LYS232
A	GLU284
A	GLY292
B	ARG98
B	ASN192

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	BINDING: BINDING => ECO:0000269|PubMed:17913711, ECO:0007744|PDB:2RD5

Chain	Residue	Details
A	LYS247
B	LYS247

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	MOD_RES: N-acetylthreonine => ECO:0007744|PubMed:22223895

Chain	Residue	Details
A	THR1
B	THR1

---

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	7
Details	M-CSA 870

Chain	Residue	Details
A	LYS41
A	GLY44
A	GLY76
A	ARG98
A	ASP196
A	ASP216
A	LYS255

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