4UQI
AP2 controls clathrin polymerization with a membrane-activated switch
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | AP-2 COMPLEX SUBUNIT ALPHA-2 | polymer | 628 | 70310.1 | 1 | UniProt (P18484) Pfam (PF01602) | RATTUS NORVEGICUS (NORWAY RAT) | 100 KDA COATED VESICLE PROTEIN C, ADAPTER-RELATED PROTEIN COMPLEX 2 SUBUNIT ALPHA-2, ADAPTOR PROTEIN COMPLEX AP-2 SUBUNIT ALPHA-2, ALPHA-ADAPTIN C, ALPHA2-ADAPTIN, CLATHRIN ASSEMBLY PROTEIN COMPLEX 2 ALPHA-C LARGE CHAIN, PLASMA MEMBRANE ADAPTOR HA2/AP2 ADAPTIN ALPHA C SUBUNIT, AP2 CLATHRIN ADAPTOR |
| 2 | B (B) | AP-2 COMPLEX SUBUNIT BETA | polymer | 657 | 73935.9 | 1 | UniProt (P63010) Pfam (PF01602) | HOMO SAPIENS (HUMAN) | AP105B, ADAPTER-RELATED PROTEIN COMPLEX 2 SUBUNIT BETA, ADAPTOR PROTEIN COMPLEX AP-2 SUBUNIT BETA, BETA-2-ADAPTIN, BETA-ADAPTIN, CLATHRIN ASSEMBLY PROTEIN COMPLEX 2 BETA LARGE CHAIN, PLASMA MEMBRANE ADAPTOR HA2/AP2 ADAPTIN BETA SUBUNIT |
| 3 | C (M) | AP-2 COMPLEX SUBUNIT MU | polymer | 446 | 51044.1 | 1 | UniProt (P84092) Pfam (PF01217) Pfam (PF00928) | RATTUS NORVEGICUS (NORWAY RAT) | AP-2 MU CHAIN, ADAPTER-RELATED PROTEIN COMPLEX 2 SUBUNIT MU, ADAPTOR PROTEIN COMPLEX AP-2 SUBUNIT MU, CLATHRIN ASSEMBLY PROTEIN COMPLEX 2 MU MEDIUM CHAIN, CLATHRIN COAT ASSEMBLY PROTEIN AP50, CLATHRIN COAT-ASSOCIATED PROTEIN AP50, MU2-ADAPTIN, PLASMA MEMBRANE ADAPTOR AP-2 50 KDA PROTEIN |
| 4 | D (S) | AP-2 COMPLEX SUBUNIT SIGMA | polymer | 142 | 17038.7 | 1 | UniProt (P62743) Pfam (PF01217) | MUS MUSCULUS (HOUSE MOUSE) | ADAPTER-RELATED PROTEIN COMPLEX 2 SUBUNIT SIGMA, ADAPTOR PROTEIN COMPLEX AP-2 SUBUNIT SIGMA, CLATHRIN ASSEMBLY PROTEIN 2 SIGMA SMALL CHAIN, CLATHRIN COAT ASSEMBLY PROTEIN AP17, CLATHRIN COAT-ASSOCIATED PROTEIN AP17, PLASMA MEMBRANE ADAPTOR AP-2 17 KDA PROTEIN, SIGMA-ADAPTIN 3B, SIGMA2-ADAPTIN |
| 5 | E (A) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 6 | F (A) | INOSITOL HEXAKISPHOSPHATE | non-polymer | 660.0 | 1 | Chemie (IHP) | |||
| 7 | G, H (A, B) | water | water | 18.0 | 3 | Chemie (HOH) |
Sequence modifications
A: 1 - 621 (UniProt: P18484)
B: 1 - 651 (UniProt: P63010)
M: 1 - 435 (UniProt: P84092)
| PDB | External Database | Details |
|---|---|---|
| Asp 8 | Glu 8 | conflict |
| Glu 272 | - | insertion |
| Gly 622 | - | expression tag |
| Ser 623 | - | expression tag |
| Gly 624 | - | expression tag |
| Leu 625 | - | expression tag |
| Val 626 | - | expression tag |
| Pro 627 | - | expression tag |
| Arg 628 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| His 652 | - | expression tag |
| His 653 | - | expression tag |
| His 654 | - | expression tag |
| His 655 | - | expression tag |
| His 656 | - | expression tag |
| His 657 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met 236 | - | insertion |
| Glu 236 | - | insertion |
| Gln 236 | - | insertion |
| Lys 236 | - | insertion |
| Leu 236 | - | insertion |
| Ile 236 | - | insertion |
| Ser 236 | - | insertion |
| Glu 236 | - | insertion |
| Glu 236 | - | insertion |
| Asp 236 | - | insertion |
| Leu 236 | - | insertion |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 212328.8 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 695.5 | |
| All* | Total formula weight | 213024.3 |
