4UQI
AP2 controls clathrin polymerization with a membrane-activated switch
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-20 |
| Detector | ADSC CCD |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 121.300, 121.300, 259.370 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 97.370 - 2.790 |
| R-factor | 0.20573 |
| Rwork | 0.203 |
| R-free | 0.25920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vgl |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.538 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 97.370 | 2.860 |
| High resolution limit [Å] | 2.790 | 2.790 |
| Rmerge | 0.070 | 0.860 |
| Number of reflections | 55666 | |
| <I/σ(I)> | 21.9 | 3.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10.9 | 11.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.2 | 18% PEG1000, 10MM NA/K PHOSPHATE PH 6.2, 200MM NACL, 4MM DTT, IP6 |
