4UNA

THE CRYSTAL STRUCTURE OF I-DMOI IN COMPLEX WITH ITS TARGET DNA AT 2 DAYS INCUBATION IN 5MM MN (STATE 4)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D, G (A, D, G)	HOMING ENDONUCLEASE I-DMOI	polymer	199	23265.1	3	UniProt (P21505) Pfam (PF14528)	DESULFUROCOCCUS MOBILIS	I-DMOI
2	B, E, H (B, E, H)	25MER	polymer	25	7707.9	3		SYNTHETIC CONSTRUCT
3	C, F, I (C, F, I)	5'-D(*CP*GP*CP*GP*CP*CP*GP*GP*AP*AP*CP*TP*TP*AP*C)-3'	polymer	15	4555.0	3		SYNTHETIC CONSTRUCT
4	J, K, L (K, M, O)	5'-D(*CP*CP*GP*GP*CP*AP*AP*GP*GP*C)-3'	polymer	10	3055.0	3		SYNTHETIC CONSTRUCT
5	M, N, O, P, Q... (A, D, G)	MANGANESE (II) ION	non-polymer		54.9	9	Chemie (MN)
6	AA, BA, CA, DA, EA... (F, G, H, I, M...)	water	water		18.0	119	Chemie (HOH)

Sequence modifications

A, D, G: 2 - 188 (UniProt: P21505)

PDB	External Database	Details
Ala 1	-	expression tag
Ala 189	-	expression tag
Ala 190	-	expression tag
Ala 191	-	expression tag
Leu 192	-	expression tag
Glu 193	-	expression tag
His 194	-	expression tag
His 195	-	expression tag
His 196	-	expression tag
His 197	-	expression tag
His 198	-	expression tag
His 199	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	115748.9
Non-Polymers*	Number of molecules	9
	Total formula weight	494.4
All*	Total formula weight	116243.3

*Water molecules are not included.