4UN9

THE CRYSTAL STRUCTURE OF I-DMOI IN COMPLEX WITH ITS TARGET DNA AT 8H INCUBATION IN 5MM MN (STATE 3)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, D)	HOMING ENDONUCLEASE I-DMOI	polymer	199	23265.1	2	UniProt (P21505) Pfam (PF14528)	DESULFUROCOCCUS MOBILIS
2	B, E, H (B, E, H)	25MER	polymer	25	7707.9	3		SYNTHETIC CONSTRUCT
3	C, F, I (C, F, I)	25MER	polymer	25	7654.9	3		SYNTHETIC CONSTRUCT
4	G (G)	HOMING ENDONUCLEASE I-DMOI	polymer	199	23280.1	1	UniProt (P21505) Pfam (PF14528)	DESULFUROCOCCUS MOBILIS
5	J, K, L, M, N... (A, D, G)	MANGANESE (II) ION	non-polymer		54.9	10	Chemie (MN)
6	AA, BA, T, U, V... (H, I, A, B, C...)	water	water		18.0	104	Chemie (HOH)

Sequence modifications

A, D: 2 - 188 (UniProt: P21505)

PDB	External Database	Details
Ala 1	-	expression tag
Ala 189	-	expression tag
Ala 190	-	expression tag
Ala 191	-	expression tag
Leu 192	-	expression tag
Glu 193	-	expression tag
His 194	-	expression tag
His 195	-	expression tag
His 196	-	expression tag
His 197	-	expression tag
His 198	-	expression tag
His 199	-	expression tag

G: 2 - 188 (UniProt: P21505)

PDB	External Database	Details
Ala 1	-	expression tag
Glu 6	Asn 6	conflict
Ala 189	-	expression tag
Ala 190	-	expression tag
Ala 191	-	expression tag
Leu 192	-	expression tag
Glu 193	-	expression tag
His 194	-	expression tag
His 195	-	expression tag
His 196	-	expression tag
His 197	-	expression tag
His 198	-	expression tag
His 199	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	115898.8
Non-Polymers*	Number of molecules	10
	Total formula weight	549.4
All*	Total formula weight	116448.1

*Water molecules are not included.