4UFS

Low resolution structure R-spondin-2 (Fu1Fu2) in complex with the ectodomains of LGR5 and ZNRF3

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	LEUCINE-RICH REPEAT-CONTAINING G-PROTEIN COUPLED RECEPTOR 5	polymer	484	54108.5	1	UniProt (O75473) Pfam (PF01462) Pfam (PF13855) Pfam (PF12799)	HOMO SAPIENS (HUMAN)	G-PROTEIN COUPLED RECEPTOR 49, G-PROTEIN COUPLED RECEPTOR 6 7, G-PROTEIN COUPLED RECEPTOR HG38, LGR5
2	B (B)	R-SPONDIN-2	polymer	120	13946.9	1	UniProt (Q8BFU0) Pfam (PF15913)	HOMO SAPIENS (HUMAN)	CYSTEINE-RICH AND SINGLE THROMBOSPONDIN DOMAIN-CONTAINING PROTEIN 2, CRISTIN-2, MCRISTIN-2, ROOF PLATE-SPECIFIC SPONDIN-2, RSPO2
3	C (C)	E3 UBIQUITIN-PROTEIN LIGASE ZNRF3	polymer	165	18203.6	1	UniProt (Q5SSZ7) Pfam (PF18212) UniProt (by SIFTS) (Q9ULT6)	HOMO SAPIENS (HUMAN)	ZINC/RING FINGER PROTEIN 3, ZNRF3

Sequence modifications

A: 32 - 487 (UniProt: O75473)

PDB	External Database	Details
Glu 29	-	expression tag
Thr 30	-	expression tag
Leu 31	-	expression tag
Gly 545	-	expression tag
Thr 546	-	expression tag
His 547	-	expression tag
His 548	-	expression tag
His 549	-	expression tag
His 550	-	expression tag
His 551	-	expression tag
His 552	-	expression tag
His 553	-	expression tag
His 554	-	expression tag
His 555	-	expression tag
His 556	-	expression tag

A: 538 - 544 (UniProt: O75473)

PDB	External Database	Details
Asn 488	-	linker
Asn 489	-	linker
Gly 490	-	linker
Asn 491	-	linker
Asn 492	-	linker
Gly 493	-	linker
Asp 494	-	linker

B: 39 - 144 (UniProt: Q8BFU0)

PDB	External Database	Details
Glu 36	-	expression tag
Thr 37	-	expression tag
Gly 38	-	expression tag
Thr 145	-	expression tag
His 146	-	expression tag
His 147	-	expression tag
His 148	-	expression tag
His 149	-	expression tag
His 150	-	expression tag
His 151	-	expression tag
His 152	-	expression tag
His 153	-	expression tag
His 154	-	expression tag
His 155	-	expression tag

C: 53 - 205 (UniProt: Q5SSZ7)

PDB	External Database	Details
Glu 50	-	expression tag
Thr 51	-	expression tag
Gly 52	-	expression tag
Gly 206	-	expression tag
Thr 207	-	expression tag
Lys 208	-	expression tag
His 209	-	expression tag
His 210	-	expression tag
His 211	-	expression tag
His 212	-	expression tag
His 213	-	expression tag
His 214	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	86258.9
All*	Total formula weight	86258.9

*Water molecules are not included.