4U4S

Crystal structure of the GluA2 ligand-binding domain (S1S2J-L483Y-N754S) in complex with glutamate and BPAM25 at 1.90 A resolution.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Glutamate receptor 2,Glutamate receptor 2	polymer	263	29244.7	2	UniProt (P19491) Pfam (PF10613) Pfam (PF00060) In PDB	Rattus norvegicus (Rat)	GluR-2,AMPA-selective glutamate receptor 2,GluR-B,GluR-K2,Glutamate receptor ionotropic,AMPA 2,GluA2,GluR-2,AMPA-selective glutamate receptor 2,GluR-B,GluR-K2,Glutamate receptor ionotropic,AMPA 2,GluA2
2	A, B	4-ethyl-3,4-dihydro-2H-pyrido[4,3-e][1,2,4]thiadiazine 1,1-dioxide	non-polymer		213.3	2	Chemie (3C1)
3	A, B	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
4	A, B	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
5	A, B	ACETATE ION	non-polymer		59.0	8	Chemie (ACT)
6	A, B	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
7	A, B	GLUTAMIC ACID	non-polymer		147.1	2	Chemie (GLU)
8	B	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
9		water	water		18.0	402	Chemie (HOH)

Sequence modifications

A, B: 392 - 506 (UniProt: P19491)

PDB	External Database	Details
Gly 390	-	expression tag
Ala 391	-	expression tag
Tyr 483	Leu 504	engineered mutation

A, B: 632 - 775 (UniProt: P19491)

PDB	External Database	Details
Gly 507	-	linker
Thr 508	-	linker
Ser 754	Asn 775	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	58489.4
Non-Polymers*	Number of molecules	22
	Total formula weight	2026.7
All*	Total formula weight	60516.1

*Water molecules are not included.