4U4C

The molecular architecture of the TRAMP complex reveals the organization and interplay of its two catalytic activities

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	ATP-dependent RNA helicase DOB1	polymer	998	113579.9	1	UniProt (P47047) Pfam (PF00270) Pfam (PF00271) Pfam (PF21408) Pfam (PF13234) Pfam (PF08148)	Saccharomyces cerevisiae (Baker's yeast)	mRNA transport regulator MTR4
2	B (B)	Protein AIR2,Poly(A) RNA polymerase protein 2	polymer	116	12830.8	1	UniProt (Q12476) UniProt (P53632)	Saccharomyces cerevisiae (Baker's yeast)	Arginine methyltransferase-interacting RING finger protein 2,DNA polymerase kappa,DNA polymerase sigma,Topoisomerase 1-related protein TRF4
3	C, D, E, F, G... (A)	SULFATE ION	non-polymer		96.1	7	Chemie (SO4)
4	J, K (A)	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
5	L, M (A)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
6	N, O (A)	TETRAETHYLENE GLYCOL	non-polymer		194.2	2	Chemie (PG4)
7	P (A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
8	Q, R (A, B)	water	water		18.0	221	Chemie (HOH)

Sequence modifications

A: 81 - 1073 (UniProt: P47047)

PDB	External Database	Details
Gly 76	-	expression tag
Ala 77	-	expression tag
Ala 78	-	expression tag
Ser 79	-	expression tag
Met 80	-	expression tag

B: 1 - 62 (UniProt: Q12476)

PDB	External Database	Details
Gly -3	-	expression tag
Ala -2	-	expression tag
Ala -1	-	expression tag
Ser 0	-	expression tag

B: 111 - 160 (UniProt: P53632)

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	126410.8
Non-Polymers*	Number of molecules	14
	Total formula weight	1362.1
All*	Total formula weight	127772.8

*Water molecules are not included.