4U4C
The molecular architecture of the TRAMP complex reveals the organization and interplay of its two catalytic activities
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-26 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 156.184, 166.768, 131.259 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.696 - 2.400 |
| R-factor | 0.2055 |
| Rwork | 0.204 |
| R-free | 0.24010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xgj |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.122 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.000 | 2.483 |
| High resolution limit [Å] | 2.400 | 2.397 |
| Rmerge | 1.075 | |
| Number of reflections | 67064 | |
| <I/σ(I)> | 15.6 | 1.16 |
| Completeness [%] | 99.6 | 96.52 |
| Redundancy | 5.6 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 285.15 | 0.1 M Tris pH 8.2, 0.15 M Li2SO4, 32% PEG 400 |
