4TXO

Crystal structure of the mixed disulfide complex of thioredoxin-like TlpAs(C110S) and copper chaperone ScoIs(C74S)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G (C, A, E, G)	Thiol:disulfide interchange protein TlpA	polymer	184	19528.6	4	UniProt (P43221) Pfam (PF08534)	Bradyrhizobium diazoefficiens USDA 110	Cytochrome c biogenesis protein TlpA
2	B, D, F, H (D, B, F, H)	Blr1131 protein	polymer	175	19325.9	4	UniProt (Q89VB6) Pfam (PF02630)	Bradyrhizobium diazoefficiens USDA 110	ScoI S
3	I, K (C, A)	THIOCYANATE ION	non-polymer		58.1	2	Chemie (SCN)
4	J (C)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
5	L, M (A, B)	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
6	N, O, P, Q, R... (C, D, A, B, E...)	water	water		18.0	590	Chemie (HOH)

Sequence modifications

C, A, E, G: 38 - 221 (UniProt: P43221)

PDB	External Database	Details
Ser 110	Cys 110	engineered mutation

D, B, F, H: 30 - 196 (UniProt: Q89VB6)

PDB	External Database	Details
Trp 22	-	expression tag
Ser 23	-	expression tag
His 24	-	expression tag
Pro 25	-	expression tag
Gln 26	-	expression tag
Phe 27	-	expression tag
Glu 28	-	expression tag
Lys 29	-	expression tag
Ser 74	Cys 74	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	155417.9
Non-Polymers*	Number of molecules	5
	Total formula weight	268.3
All*	Total formula weight	155686.2

*Water molecules are not included.