4TSM
MBP-fusion protein of PilA1 from C. difficile R20291 residues 26-166
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C | maltose-binding protein, pilin chimera | polymer | 520 | 56380.5 | 3 | UniProt (D8A942) UniProt (C9YRY2) Pfam (PF01547) UniProt (by SIFTS) (P0AEX9) In PDB | Escherichia coli | MBP, Mmbp,PilA1 |
2 | D, E, F | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 666.6 | 3 | In PDB BIRD (PRD_900010) GlyTouCan (G87171PZ) | alpha-maltotetraose | ||
3 | A, B, C | trimethylamine oxide | non-polymer | 75.1 | 19 | Chemie (TMO) | |||
4 | water | water | 18.0 | 797 | Chemie (HOH) |
Sequence modifications
A, B, C: 1 - 366 (UniProt: D8A942)
A, B, C: 1026 - 1164 (UniProt: C9YRY2)
PDB | External Database | Details |
---|---|---|
Met 0 | - | initiating methionine |
Ala 82 | Asp 108 | engineered mutation |
Ala 83 | Lys 109 | engineered mutation |
Ala 172 | Glu 198 | engineered mutation |
Ala 173 | Asn 199 | engineered mutation |
Ala 239 | Lys 265 | engineered mutation |
Ala 362 | Lys 388 | engineered mutation |
Ala 363 | Asp 389 | engineered mutation |
Asn 367 | - | linker |
Ala 368 | - | linker |
Ala 369 | - | linker |
Ala 370 | - | linker |
PDB | External Database | Details |
---|---|---|
Gly 1165 | - | expression tag |
Ser 1166 | - | expression tag |
Leu 1167 | - | expression tag |
Glu 1168 | - | expression tag |
His 1169 | - | expression tag |
His 1170 | - | expression tag |
His 1171 | - | expression tag |
His 1172 | - | expression tag |
His 1173 | - | expression tag |
His 1174 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 3 |
Total formula weight | 169141.5 | |
Branched | Number of molecules | 3 |
Total formula weight | 1999.7 | |
Non-Polymers* | Number of molecules | 19 |
Total formula weight | 1427.1 | |
All* | Total formula weight | 172568.4 |