4TSM
MBP-fusion protein of PilA1 from C. difficile R20291 residues 26-166
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 99.1 |
Detector technology | PIXEL |
Collection date | 2014-06-10 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979470 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 143.951, 79.366, 164.746 |
Unit cell angles | 90.00, 90.23, 90.00 |
Refinement procedure
Resolution | 38.580 - 1.899 |
Rwork | 0.197 |
R-free | 0.23260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ogm |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.580 | 1.966 |
High resolution limit [Å] | 1.899 | 1.899 |
Rmerge | 0.178 | 1.844 |
Number of reflections | 139534 | |
<I/σ(I)> | 1.89 | 1.14 |
Completeness [%] | 94.9 | 92.48 |
Redundancy | 7.1 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 20% PEG2000 MME, 0.2 M trimethylamine N-oxide, 0.1 M Tris-HCl, pH 8.5 |