4TRU
Crystal structure of Q24A Q25A mutant of human macrophage migration inhibitory factor
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C (A, B, C) | Macrophage migration inhibitory factor | polymer | 114 | 12241.0 | 3 | UniProt (P14174) Pfam (PF01187) | Homo sapiens (Human) | MIF,Glycosylation-inhibiting factor,GIF,L-dopachrome isomerase,L-dopachrome tautomerase,Phenylpyruvate tautomerase |
| 2 | D, G, J (A, B, C) | SULFATE ION | non-polymer | 96.1 | 3 | Chemie (SO4) | |||
| 3 | E, H, K (A, B, C) | ISOPROPYL ALCOHOL | non-polymer | 60.1 | 3 | Chemie (IPA) | |||
| 4 | F, I (A, B) | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
| 5 | L, M, N (A, B, C) | water | water | 18.0 | 316 | Chemie (HOH) |
Sequence modifications
A, B, C: 1 - 114 (UniProt: P14174)
| PDB | External Database | Details |
|---|---|---|
| Ala 24 | Gln 25 | engineered mutation |
| Ala 25 | Gln 26 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 36722.9 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 652.7 | |
| All* | Total formula weight | 37375.5 |
