4S3M

Evidence of kinetic cooperativity in dimeric Ketopantoate Reductase from Staphylococcus aureus

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	2-dehydropantoate 2-reductase	polymer	294	33478.0	2	UniProt (Q99R37) Pfam (PF02558) Pfam (PF08546) UniProt (by SIFTS) (A0A0J9X201)	Staphylococcus aureus subsp. aureus Mu50
2	C, D (A, B)	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	non-polymer		743.4	2	Chemie (NAP)
3	E, F (A, B)	water	water		18.0	42	Chemie (HOH)

Sequence modifications

A, B: 3 - 286 (UniProt: Q99R37)

PDB	External Database	Details
Met 1	-	expression tag
Ser 2	-	expression tag
Leu 181	Ala 180	engineered mutation
Glu 287	-	expression tag
Gly 288	-	expression tag
His 289	-	expression tag
His 290	-	expression tag
His 291	-	expression tag
His 292	-	expression tag
His 293	-	expression tag
His 294	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	66956.0
Non-Polymers*	Number of molecules	2
	Total formula weight	1486.8
All*	Total formula weight	68442.8

*Water molecules are not included.