4RYU

Crystal Structure of C2 form of E112A Mutant of Stationary Phase Survival Protein (SurE) from Salmonella typhimurium

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	5'/3'-nucleotidase SurE	polymer	267	28557.1	4	UniProt (P66881) Pfam (PF01975)	Salmonella enterica subsp. enterica serovar Enteritidis	Exopolyphosphatase, Nucleoside monophosphate phosphohydrolase
2	E, F, G, H, I... (A, B, C, D)	PHOSPHATE ION	non-polymer		95.0	8	Chemie (PO4)
3	J, L, R, W (A, B, C, D)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
4	M, S, T, X (B, C, D)	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
5	N, O, P, U, Y (B, C, D)	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	5	Chemie (MPD)
6	AA, BA, CA, Z (B, C, D, A)	water	water		18.0	933	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 253 (UniProt: P66881)

PDB	External Database	Details
Met -13	-	expression tag
Arg -12	-	expression tag
Gly -11	-	expression tag
Ser -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Met -2	-	expression tag
Ala -1	-	expression tag
Ser 0	-	expression tag
Ala 112	Glu 112	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	114228.3
Non-Polymers*	Number of molecules	21
	Total formula weight	1816.2
All*	Total formula weight	116044.5

*Water molecules are not included.