4RYU
Crystal Structure of C2 form of E112A Mutant of Stationary Phase Survival Protein (SurE) from Salmonella typhimurium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-04-11 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 160.390, 95.780, 94.230 |
| Unit cell angles | 90.00, 100.06, 90.00 |
Refinement procedure
| Resolution | 48.000 - 2.040 |
| R-factor | 0.17991 |
| Rwork | 0.178 |
| R-free | 0.21415 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v4n |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.365 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.870 | 2.140 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Number of reflections | 89024 | |
| <I/σ(I)> | 11.5 | 2.3 |
| Completeness [%] | 98.6 | 91 |
| Redundancy | 4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 293 | 0.2 M Sodium citrate tribasic dihydrate, 0.1 M HEPES (pH 7.5), 30% 2-Methyl 2,4-pentanediol, Under Oil, Microbatch method, temperature 293K |
