4RQ9

Crystal structure of the chromophore-binding domain of Stigmatella aurantiaca bacteriophytochrome (Thr289His mutant) in the Pr state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Photoreceptor-histidine kinase BphP	polymer	348	38851.0	1	UniProt (Q097N3) Pfam (PF08446) Pfam (PF01590) In PDB	Stigmatella aurantiaca	Sensor protein
2	A	BILIVERDINE IX ALPHA	non-polymer		582.6	1	Chemie (BLA)
3	A	S,R MESO-TARTARIC ACID	non-polymer		150.1	1	Chemie (SRT)
4	A	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
5	A	GLYCEROL	non-polymer		92.1	15	Chemie (GOL)
6		water	water		18.0	178	Chemie (HOH)

Sequence modifications

A: 1 - 328 (UniProt: Q097N3)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
His 289	Thr 289	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	38851.0
Non-Polymers*	Number of molecules	18
	Total formula weight	2176.2
All*	Total formula weight	41027.2

*Water molecules are not included.