4RQ9
Crystal structure of the chromophore-binding domain of Stigmatella aurantiaca bacteriophytochrome (Thr289His mutant) in the Pr state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 131.813, 131.813, 97.218 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.960 - 2.500 |
| R-factor | 0.1566 |
| Rwork | 0.155 |
| R-free | 0.17776 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4RPW |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.858 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.085 | 0.543 |
| Number of reflections | 33975 | |
| <I/σ(I)> | 19.3 | 3.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 289 | protein: 25-28 mg ml-1 in 10 mM Tris HCl (pH 8.0) and 10 mM NaCl. crystallization conditions: 260 mM potassium sodium tartrate tetrahydrate, 35% (v/v) glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
