4RQ8
Human DNA Polymerase Beta With Gapped DNA Containing an 8-oxo-7,8-dihydro-Guanine(8-oxoG) and dATP soaked with MnCl2 for 35 s
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA polymerase beta | polymer | 343 | 39258.8 | 1 | UniProt (P06746) Pfam (PF14716) Pfam (PF10391) Pfam (PF14792) Pfam (PF14791) | Homo sapiens (human) | |
| 2 | B (D) | DNA (5'-D(P*GP*TP*CP*GP*G)-3') | polymer | 5 | 1536.0 | 1 | synthetic construct | 5-mer phosphorylated Downstream Primer | |
| 3 | C (P) | DNA (5'-D(*GP*CP*TP*GP*AP*TP*GP*CP*GP*CP*A)-3') | polymer | 11 | 3374.2 | 1 | synthetic construct | 11-mer Primer | |
| 4 | D (T) | DNA (5'-D(*CP*CP*GP*AP*CP*(8OG)P*GP*CP*GP*CP*AP*TP*CP*AP*GP*C)-3') | polymer | 16 | 4885.2 | 1 | synthetic construct | 16-mer Template | |
| 5 | E (A) | PYROPHOSPHATE | non-polymer | 178.0 | 1 | Chemie (PPV) | |||
| 6 | CA, F, G, H, I... (P, A) | MANGANESE (II) ION | non-polymer | 54.9 | 6 | Chemie (MN) | |||
| 7 | DA, EA, K, L, M... (T, A) | SODIUM ION | non-polymer | 23.0 | 9 | Chemie (NA) | |||
| 8 | R, S, T, U, V... (A) | CHLORIDE ION | non-polymer | 35.5 | 6 | Chemie (CL) | |||
| 9 | AA, BA, FA, X, Y... (A, T) | ACETATE ION | non-polymer | 59.0 | 6 | Chemie (ACT) | |||
| 10 | GA, HA, IA, JA (A, D, P, T) | water | water | 18.0 | 231 | Chemie (HOH) |
Sequence modifications
A: 1 - 335 (UniProt: P06746)
| PDB | External Database | Details |
|---|---|---|
| Met -1 | - | expression tag |
| Gly 0 | - | expression tag |
| His 336 | - | expression tag |
| His 337 | - | expression tag |
| His 338 | - | expression tag |
| His 339 | - | expression tag |
| His 340 | - | expression tag |
| His 341 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 49054.2 | |
| Non-Polymers* | Number of molecules | 28 |
| Total formula weight | 1281.5 | |
| All* | Total formula weight | 50335.7 |
