4RQ8
Human DNA Polymerase Beta With Gapped DNA Containing an 8-oxo-7,8-dihydro-Guanine(8-oxoG) and dATP soaked with MnCl2 for 35 s
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0001701 | biological_process | in utero embryonic development |
| A | 0003677 | molecular_function | DNA binding |
| A | 0003684 | molecular_function | damaged DNA binding |
| A | 0003887 | molecular_function | DNA-directed DNA polymerase activity |
| A | 0003906 | molecular_function | DNA-(apurinic or apyrimidinic site) endonuclease activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005634 | cellular_component | nucleus |
| A | 0005654 | cellular_component | nucleoplasm |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005874 | cellular_component | microtubule |
| A | 0005876 | cellular_component | spindle microtubule |
| A | 0006259 | biological_process | DNA metabolic process |
| A | 0006260 | biological_process | DNA replication |
| A | 0006261 | biological_process | DNA-templated DNA replication |
| A | 0006281 | biological_process | DNA repair |
| A | 0006284 | biological_process | base-excision repair |
| A | 0006287 | biological_process | base-excision repair, gap-filling |
| A | 0006290 | biological_process | pyrimidine dimer repair |
| A | 0006297 | biological_process | nucleotide-excision repair, DNA gap filling |
| A | 0006303 | biological_process | double-strand break repair via nonhomologous end joining |
| A | 0006915 | biological_process | apoptotic process |
| A | 0006954 | biological_process | inflammatory response |
| A | 0006974 | biological_process | DNA damage response |
| A | 0007435 | biological_process | salivary gland morphogenesis |
| A | 0008017 | molecular_function | microtubule binding |
| A | 0008630 | biological_process | intrinsic apoptotic signaling pathway in response to DNA damage |
| A | 0009059 | biological_process | macromolecule biosynthetic process |
| A | 0010332 | biological_process | response to gamma radiation |
| A | 0016445 | biological_process | somatic diversification of immunoglobulins |
| A | 0016446 | biological_process | somatic hypermutation of immunoglobulin genes |
| A | 0016779 | molecular_function | nucleotidyltransferase activity |
| A | 0016829 | molecular_function | lyase activity |
| A | 0019899 | molecular_function | enzyme binding |
| A | 0032991 | cellular_component | protein-containing complex |
| A | 0034061 | molecular_function | DNA polymerase activity |
| A | 0045471 | biological_process | response to ethanol |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0048535 | biological_process | lymph node development |
| A | 0048536 | biological_process | spleen development |
| A | 0048872 | biological_process | homeostasis of number of cells |
| A | 0051402 | biological_process | neuron apoptotic process |
| A | 0051575 | molecular_function | 5'-deoxyribose-5-phosphate lyase activity |
| A | 0055093 | biological_process | response to hyperoxia |
| A | 0071707 | biological_process | immunoglobulin heavy chain V-D-J recombination |
| A | 0071897 | biological_process | DNA biosynthetic process |
| A | 0140078 | molecular_function | class I DNA-(apurinic or apyrimidinic site) endonuclease activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 16 |
| Details | BINDING SITE FOR RESIDUE PPV A 401 |
| Chain | Residue |
| A | ARG149 |
| A | HOH502 |
| A | HOH512 |
| A | HOH575 |
| A | HOH589 |
| P | DA11 |
| P | MN101 |
| P | HOH501 |
| A | GLY179 |
| A | SER180 |
| A | ARG183 |
| A | SER188 |
| A | GLY189 |
| A | ASP190 |
| A | ASP192 |
| A | MN404 |
| site_id | AC2 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE MN A 402 |
| Chain | Residue |
| A | ASP190 |
| A | ASP192 |
| A | ASP256 |
| A | MN404 |
| A | HOH501 |
| P | DC10 |
| P | DA11 |
| site_id | AC3 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE MN A 403 |
| Chain | Residue |
| A | GLU9 |
| A | HIS337 |
| A | HIS339 |
| A | HOH519 |
| A | HOH520 |
| A | HOH521 |
| A | HOH522 |
| site_id | AC4 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE MN A 404 |
| Chain | Residue |
| A | ASP190 |
| A | ASP192 |
| A | PPV401 |
| A | MN402 |
| A | HOH502 |
| P | DA11 |
| site_id | AC5 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MN A 405 |
| Chain | Residue |
| A | GLU117 |
| A | ASP321 |
| A | HOH639 |
| A | HOH660 |
| A | HOH669 |
| site_id | AC6 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE MN A 406 |
| Chain | Residue |
| A | LYS48 |
| A | HIS336 |
| A | HIS338 |
| A | HOH659 |
| site_id | AC7 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE NA A 407 |
| Chain | Residue |
| A | HIS340 |
| A | HOH525 |
| A | HOH526 |
| A | HOH666 |
| site_id | AC8 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE NA A 408 |
| Chain | Residue |
| A | LYS60 |
| A | LEU62 |
| A | VAL65 |
| A | HOH547 |
| A | HOH548 |
| D | DC3 |
| site_id | AC9 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE NA A 409 |
| Chain | Residue |
| A | THR101 |
| A | VAL103 |
| A | ILE106 |
| A | HOH553 |
| P | DG9 |
| P | HOH504 |
| site_id | BC1 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE NA A 410 |
| Chain | Residue |
| A | HIS285 |
| A | GLU288 |
| A | HOH670 |
| site_id | BC2 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE NA A 411 |
| Chain | Residue |
| A | ASP130 |
| A | ASP314 |
| A | HOH656 |
| A | HOH657 |
| site_id | BC3 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE NA A 412 |
| Chain | Residue |
| A | ASP145 |
| A | HIS252 |
| A | HOH585 |
| A | HOH586 |
| A | HOH596 |
| site_id | BC4 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE NA A 413 |
| Chain | Residue |
| A | ASP160 |
| A | HOH594 |
| A | HOH595 |
| site_id | BC5 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE CL A 414 |
| Chain | Residue |
| A | ASN294 |
| A | THR297 |
| A | ARG299 |
| site_id | BC6 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE CL A 415 |
| Chain | Residue |
| A | ALA32 |
| A | ILE33 |
| A | HIS34 |
| A | LYS35 |
| site_id | BC7 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE CL A 416 |
| Chain | Residue |
| A | ARG183 |
| A | SER334 |
| A | GLU335 |
| A | HOH513 |
| site_id | BC8 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE CL A 417 |
| Chain | Residue |
| A | ASN281 |
| site_id | BC9 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE CL A 418 |
| Chain | Residue |
| A | HIS34 |
| A | ASN37 |
| A | ACT420 |
| A | HOH532 |
| T | DC5 |
| site_id | CC1 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE CL A 419 |
| Chain | Residue |
| A | LEU82 |
| A | ARG89 |
| site_id | CC2 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE ACT A 420 |
| Chain | Residue |
| A | CL418 |
| D | DG1 |
| T | DC5 |
| T | HOH202 |
| A | ASN37 |
| A | ALA38 |
| A | LYS41 |
| A | LYS280 |
| site_id | CC3 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE ACT A 421 |
| Chain | Residue |
| A | HIS34 |
| A | HOH653 |
| T | DC5 |
| site_id | CC4 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE ACT A 422 |
| Chain | Residue |
| A | TYR296 |
| site_id | CC5 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE ACT A 423 |
| Chain | Residue |
| A | GLN219 |
| A | THR225 |
| A | ASP226 |
| A | THR227 |
| A | HOH574 |
| site_id | CC6 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE ACT A 424 |
| Chain | Residue |
| A | GLU9 |
| site_id | CC7 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MN P 101 |
| Chain | Residue |
| A | PPV401 |
| A | HOH575 |
| P | DA11 |
| P | HOH501 |
| P | HOH509 |
| site_id | CC8 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE NA T 101 |
| Chain | Residue |
| T | 8OG6 |
| T | DG7 |
| T | HOH204 |
| T | HOH205 |
| T | HOH227 |
| site_id | CC9 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE NA T 102 |
| Chain | Residue |
| T | DA11 |
| T | HOH218 |
| T | HOH219 |
| T | HOH220 |
| T | HOH221 |
| site_id | DC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ACT T 103 |
| Chain | Residue |
| D | DC3 |
| D | DG4 |
| T | DA4 |
| T | DC5 |
Functional Information from PROSITE/UniProt
| site_id | PS00522 |
| Number of Residues | 20 |
| Details | DNA_POLYMERASE_X DNA polymerase family X signature. GSFrRGaesSgDMDVLLthP |
| Chain | Residue | Details |
| A | GLY179-PRO198 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | ACT_SITE: Nucleophile; Schiff-base intermediate with DNA; for 5'-dRP lyase activity => ECO:0000269|PubMed:9572863 |
| Chain | Residue | Details |
| A | LYS72 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:12517346, ECO:0000269|PubMed:8841120, ECO:0000269|PubMed:9287163, ECO:0007744|PDB:1BPX, ECO:0007744|PDB:1BPZ, ECO:0007744|PDB:1MQ3, ECO:0007744|PDB:1ZQO, ECO:0007744|PDB:1ZQQ |
| Chain | Residue | Details |
| A | THR101 | |
| A | VAL103 | |
| A | ILE106 | |
| A | LYS60 | |
| A | LEU62 | |
| A | VAL65 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 3 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:8841119, ECO:0007744|PDB:8ICW, ECO:0007744|PDB:8ICX, ECO:0007744|PDB:8ICY |
| Chain | Residue | Details |
| A | SER180 | |
| A | GLY189 | |
| A | ARG149 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:8841119, ECO:0007744|PDB:8ICX |
| Chain | Residue | Details |
| A | ARG183 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 3 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:9287163, ECO:0007744|PDB:1BPY |
| Chain | Residue | Details |
| A | ASP192 | |
| A | ASP256 | |
| A | ASP190 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 1 |
| Details | MOD_RES: N6-acetyllysine => ECO:0000250|UniProtKB:Q8K409 |
| Chain | Residue | Details |
| A | LYS72 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 2 |
| Details | MOD_RES: Omega-N-methylarginine; by PRMT6 => ECO:0000269|PubMed:16600869 |
| Chain | Residue | Details |
| A | ARG83 | |
| A | ARG152 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 4 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:19713937, ECO:0000269|PubMed:21362556 |
| Chain | Residue | Details |
| A | LYS41 | |
| A | LYS61 | |
| A | LYS81 |
