4RQ4

Human DNA Polymerase Beta With Gapped DNA Containing an 8-oxo-7,8-dihydro-Guanine(8-oxoG) and dATP soaked with MgCl2 for 30 s

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA polymerase beta	polymer	343	39258.8	1	UniProt (P06746) Pfam (PF14716) Pfam (PF10391) Pfam (PF14792) Pfam (PF14791)	Homo sapiens (human)
2	B (D)	DNA (5'-D(P*GP*TP*CP*GP*G)-3')	polymer	5	1536.0	1		synthetic construct	5-mer phosphorylated Downstream Primer
3	C (P)	DNA (5'-D(*GP*CP*TP*GP*AP*TP*GP*CP*GP*CP*A)-3')	polymer	11	3374.2	1		synthetic construct	10-mer Primer
4	D (T)	DNA (5'-D(*CP*CP*GP*AP*CP*(8OG)P*GP*CP*GP*CP*AP*TP*CP*AP*GP*C)-3')	polymer	16	4885.2	1		synthetic construct	16-mer Template
5	E (A)	2'-DEOXYADENOSINE 5'-TRIPHOSPHATE	non-polymer		491.2	1	Chemie (DTP)
6	F, G, Q (A, P)	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
7	H, I (A)	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
8	J, K, L, M, N (A)	CHLORIDE ION	non-polymer		35.5	5	Chemie (CL)
9	O (A)	ACETATE ION	non-polymer		59.0	1	Chemie (ACT)
10	P (A)	PYROPHOSPHATE	non-polymer		178.0	1	Chemie (PPV)
11	R, S, T, U (A, D, P, T)	water	water		18.0	195	Chemie (HOH)

Sequence modifications

A: 1 - 335 (UniProt: P06746)

PDB	External Database	Details
Met -1	-	expression tag
Gly 0	-	expression tag
His 336	-	expression tag
His 337	-	expression tag
His 338	-	expression tag
His 339	-	expression tag
His 340	-	expression tag
His 341	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	49054.2
Non-Polymers*	Number of molecules	13
	Total formula weight	1024.4
All*	Total formula weight	50078.5

*Water molecules are not included.