4RQ4
Human DNA Polymerase Beta With Gapped DNA Containing an 8-oxo-7,8-dihydro-Guanine(8-oxoG) and dATP soaked with MgCl2 for 30 s
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Detector technology | CCD |
Collection date | 2014-07-23 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.97931 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.160, 79.650, 55.310 |
Unit cell angles | 90.00, 106.35, 90.00 |
Refinement procedure
Resolution | 40.590 - 2.100 |
R-factor | 0.1923 |
Rwork | 0.190 |
R-free | 0.24363 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.342 |
Data reduction software | MOSFLM |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.590 | 2.160 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.076 | 0.382 |
Number of reflections | 23899 | |
<I/σ(I)> | 7.8 | 2.5 |
Completeness [%] | 99.7 | 99.8 |
Redundancy | 3.2 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 50 MM IMIDAZOLE, 350 MM SODIUM Acetate, 17% PEG3350, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |