4ROE

Human TFIIB-related factor 2 (Brf2) and TBP bound to RPPH1 promoter

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Transcription factor IIIB 50 kDa subunit	polymer	360	40203.4	1	UniProt (Q9HAW0) Pfam (PF21886) In PDB	Homo sapiens (human)	TFIIIB50, hTFIIIB50, B-related factor 2, BRF-2, hBRFU
2	B	TATA-box-binding protein	polymer	183	20597.4	1	UniProt (P20226) Pfam (PF00352) In PDB	Homo sapiens (human)	TATA sequence-binding protein, TATA-binding factor, TATA-box factor, Transcription initiation factor TFIID TBP subunit
3	N	Template strand	polymer	28	8525.6	1		synthetic construct
4	T	Non-template strand	polymer	28	8680.6	1		synthetic construct
5	N, T	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6		water	water		18.0	312	Chemie (HOH)

Sequence modifications

A: 62 - 419 (UniProt: Q9HAW0)

PDB	External Database	Details
Gly 60	-	expression tag
Pro 61	-	expression tag

B: 159 - 339 (UniProt: P20226)

PDB	External Database	Details
Gly 157	-	expression tag
Pro 158	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	78007.0
Non-Polymers*	Number of molecules	2
	Total formula weight	48.6
All*	Total formula weight	78055.6

*Water molecules are not included.