4RE1

Crystal structure of human TEAD1 and disulfide-engineered YAP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Transcriptional enhancer factor TEF-1	polymer	220	25509.1	2	UniProt (P28347) Pfam (PF17725)	Homo sapiens (human)	NTEF-1, Protein GT-IIC, TEA domain family member 1, TEAD-1, Transcription factor 13, TCF-13
2	C, D (C, D)	Yorkie homolog	polymer	149	15977.8	2	UniProt (P46937) Pfam (PF15238)	Homo sapiens (human)	65 kDa Yes-associated protein, YAP65
3	E (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
4	F, G, H (A, B, D)	water	water		18.0	95	Chemie (HOH)

Sequence modifications

A, B: 194 - 411 (UniProt: P28347)

PDB	External Database	Details
Met 192	-	expression tag
Ala 193	-	expression tag

C, D: 50 - 171 (UniProt: P46937)

PDB	External Database	Details
Met 23	-	expression tag
Gly 24	-	expression tag
Ser 25	-	expression tag
Ser 26	-	expression tag
His 27	-	expression tag
His 28	-	expression tag
His 29	-	expression tag
His 30	-	expression tag
His 31	-	expression tag
His 32	-	expression tag
His 33	-	expression tag
His 34	-	expression tag
His 35	-	expression tag
His 36	-	expression tag
Ser 37	-	expression tag
Ser 38	-	expression tag
Gly 39	-	expression tag
Arg 40	-	expression tag
Glu 41	-	expression tag
Asn 42	-	expression tag
Leu 43	-	expression tag
Tyr 44	-	expression tag
Phe 45	-	expression tag
Gln 46	-	expression tag
Ser 47	-	expression tag
His 48	-	expression tag
Met 49	-	expression tag
Cys 87	Arg 87	engineered mutation
Ala 93	Asp 93	engineered mutation
Cys 96	Phe 96	engineered mutation
Arg 100	Glu 100	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	82973.8
Non-Polymers*	Number of molecules	1
	Total formula weight	35.5
All*	Total formula weight	83009.3

*Water molecules are not included.