4R9L

Structure of a thermostable elevenfold mutant of limonene epoxide hydrolase from Rhodococcus erythropolis, containing two stabilizing disulfide bonds

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	Limonene-1,2-epoxide hydrolase	polymer	174	19508.2	3	UniProt (Q9ZAG3)	Rhodococcus erythropolis
2	D, E, F (A, B, C)	(2R)-2-hydroxyhexanamide	non-polymer		131.2	3	Chemie (HYH)
3	G, H, I (A, B, C)	water	water		18.0	209	Chemie (HOH)

Sequence modifications

A, B, C: 3 - 149 (UniProt: Q9ZAG3)

PDB	External Database	Details
Met 1	-	expression tag
Ala 2	-	expression tag
Cys 5	Ile 5	engineered mutation
Pro 15	Ser 15	engineered mutation
Lys 19	Ala 19	engineered mutation
Lys 76	Thr 76	engineered mutation
Cys 84	Glu 84	engineered mutation
Val 85	Thr 85	engineered mutation
Cys 89	Gly 89	engineered mutation
Cys 91	Ser 91	engineered mutation
Lys 92	Asn 92	engineered mutation
Phe 96	Tyr 96	engineered mutation
Asp 124	Glu 124	engineered mutation
Lys 150	-	expression tag
Leu 151	-	expression tag
Gly 152	-	expression tag
Pro 153	-	expression tag
Glu 154	-	expression tag
Gln 155	-	expression tag
Lys 156	-	expression tag
Leu 157	-	expression tag
Ile 158	-	expression tag
Ser 159	-	expression tag
Glu 160	-	expression tag
Glu 161	-	expression tag
Asp 162	-	expression tag
Leu 163	-	expression tag
Asn 164	-	expression tag
Ser 165	-	expression tag
Ala 166	-	expression tag
Val 167	-	expression tag
Asp 168	-	expression tag
His 169	-	expression tag
His 170	-	expression tag
His 171	-	expression tag
His 172	-	expression tag
His 173	-	expression tag
His 174	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	58524.5
Non-Polymers*	Number of molecules	3
	Total formula weight	393.5
All*	Total formula weight	58918.0

*Water molecules are not included.