4R9L

Structure of a thermostable elevenfold mutant of limonene epoxide hydrolase from Rhodococcus erythropolis, containing two stabilizing disulfide bonds

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0016787	molecular_function	hydrolase activity
A	0018744	molecular_function	limonene-1,2-epoxide hydrolase activity
B	0016787	molecular_function	hydrolase activity
B	0018744	molecular_function	limonene-1,2-epoxide hydrolase activity
C	0016787	molecular_function	hydrolase activity
C	0018744	molecular_function	limonene-1,2-epoxide hydrolase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE HYH A 201

Chain	Residue
A	ARG99
A	ASP101
A	ASP132
A	PHE134
A	HOH353
A	HOH354

---

site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE HYH B 201

Chain	Residue
B	ASP132
B	PHE139
B	HOH305
B	ARG99
B	ASP101
B	LEU103

---

site_id	AC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE HYH C 201

Chain	Residue
C	ARG99
C	ASP101
C	ASP132
C	PHE134
C	HOH303

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	3
Details	Active site: {"description":"Proton donor","evidences":[{"evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI2
Number of Residues	3
Details	Active site: {"description":"Proton acceptor","evidences":[{"evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	5
Details	M-CSA 644

Chain	Residue	Details
A	TYR53	electrostatic stabiliser
A	ASN55	electrostatic stabiliser
A	ARG99	electrostatic stabiliser, proton acceptor, proton donor, proton relay
A	ASP101	proton acceptor, proton donor
A	ASP132	activator, proton acceptor, proton donor

---

site_id	MCSA2
Number of Residues	5
Details	M-CSA 644

Chain	Residue	Details
B	TYR53	electrostatic stabiliser
B	ASN55	electrostatic stabiliser
B	ARG99	electrostatic stabiliser, proton acceptor, proton donor, proton relay
B	ASP101	proton acceptor, proton donor
B	ASP132	activator, proton acceptor, proton donor

---

site_id	MCSA3
Number of Residues	5
Details	M-CSA 644

Chain	Residue	Details
C	TYR53	electrostatic stabiliser
C	ASN55	electrostatic stabiliser
C	ARG99	electrostatic stabiliser, proton acceptor, proton donor, proton relay
C	ASP101	proton acceptor, proton donor
C	ASP132	activator, proton acceptor, proton donor

---