4R6B

Rational Design of Enhanced Photoresistance in a Photoswitchable Fluorescent Protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Green to red photoconvertible GFP-like protein EosFP	polymer	232	26481.9	4	UniProt (Q5S6Z9) Pfam (PF01353)	Lobophyllia hemprichii (Lobed brain coral)
2	E, F, G, I, K... (A, B, C, D)	SULFATE ION	non-polymer		96.1	15	Chemie (SO4)
3	H, J, Q, W (A, C, D)	SULFITE ION	non-polymer		80.1	4	Chemie (SO3)
4	AA, X, Y, Z (D, A, B, C)	water	water		18.0	822	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 226 (UniProt: Q5S6Z9)

PDB	External Database	Details
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Cr8 64	His 62	chromophore
Cr8 64	Tyr 63	chromophore
Cr8 64	Gly 64	chromophore
Ala 159	Met 159	engineered mutation
Ser 173	Phe 173	engineered mutation
Leu 191	Phe 191	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	105927.6
Non-Polymers*	Number of molecules	19
	Total formula weight	1761.2
All*	Total formula weight	107688.8

*Water molecules are not included.