4R6B
Rational Design of Enhanced Photoresistance in a Photoswitchable Fluorescent Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.93928 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 86.701, 96.350, 139.426 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.110 - 2.000 |
| R-factor | 0.2022 |
| Rwork | 0.192 |
| R-free | 0.22430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vvh |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.180 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.110 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.110 | 0.468 |
| Number of reflections | 78818 | |
| <I/σ(I)> | 14.64 | 3 |
| Completeness [%] | 96.4 | 96.9 |
| Redundancy | 4.8 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 293 | 2.1M ammonium sulfate, 0.1M Bicine, pH 8.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
